Alzheimer’s disease is one of the most common causes of dementia. Acetylcholinesterase has been considered as the main therapeutic target in the treatment of Alzheimer’s disease. Natural products are the richest source of lead compounds for the discovery and development of new drugs. In the present contribution, the hierarchical biology–oriented method was applied to discover the active gradients of F. pseudalliacea root as AChE inhibitors. The Kamonolol acetate was extracted and identified from the n-hexane extract of F. pseudalliacea root. The Kamonolol acetate inhibited the AChE with IC50: 63.9 μM. Molecular modeling studies showed that Kamonolol acetate interacted with CAS and PAS of AChE active site. Kinetics in accompany with molecular modeling studies demonstrated that Kamonolol acetate inhibited AChE in the mixed-type model. The results indicated that Kamonolol acetate could be considered as a valuable lead compound in the design of AChE inhibitors.

中文翻译：

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来自 Ferula pseudalliacea 的醋酸卡莫洛尔作为 AChE 抑制剂：体外和计算机研究

阿尔茨海默病是痴呆症的最常见原因之一。乙酰胆碱酯酶已被认为是治疗阿尔茨海默病的主要治疗靶点。天然产物是发现和开发新药的先导化合物的最丰富来源。在目前的贡献中，应用分层生物学导向的方法来发现 F. pseudalliacea 根作为 AChE 抑制剂的活性梯度。从假单胞菌根的正己烷提取物中提取并鉴定出醋酸卡蒙洛尔。醋酸卡莫洛尔抑制 AChE，IC50：63.9 μM。分子模型研究表明，醋酸卡莫洛尔与 AChE 活性位点的 CAS 和 PAS 相互作用。动力学和分子建模研究表明，醋酸卡莫洛尔在混合型模型中抑制了 AChE。