AbstractNowadays, wound dressings serve as advanced skin products, which mainly aim to accelerate the wound healing process. The wound dressings with a potential of localized and prolonged release of therapeutic agents have attracted tremendous attention. In this study, synthesis of a sustained release of niosomal Aloe vera (AV) loaded in alginate/gelatin (AG) hybrid hydrogel is aimed at improving skin regeneration as wound dressing. For this purpose, AV-loaded niosomes are synthesized and incorporated in the hybrid AG hydrogel. The size and polydispersity index (PdI) of niosomes, AV entrapment efficacy and AV in vitro release are characterized. In addition, the hydrogel characteristic, such as swelling ratio, degradation behavior and mechanical property, are studied. MTT assay is utilized to evaluate the effect of AV incorporation and release on the proliferation of fibroblast cells. Results demonstrate that size, PdI and EE% of AV-loaded niosomes are 270.080 nm, 0.108 and 42.039 ± 4.090%, respectively, and in vitro release of AV is about 20% after 7 days. AG hybrid hydrogel loaded by niosomal AV shows an extended sustained release manner, where its swelling ratio and percentage of degradation are 60 wt% after 72 h and 70 wt% after 6 days, respectively. The average Young’s modulus of the hybrid hydrogel is measured around 12.64 ± 1.3 kPa, which seems suitable as a wound dressing. Finally, MTT assay confesses an increased fibroblast proliferation in the presence of AV, particularly in the niosomal AV-loaded hydrogel. Concludingly, alginate/gelation hybrid hydrogel incorporated with niosomal AV, with sustained release potential can be suggested as a promising candidate for wound dressing applications. Graphical abstract

中文翻译：

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研究混合海藻酸盐/明胶水凝胶掺入的 niosomal 芦荟能够持续释放芦荟作为潜在的皮肤伤口敷料

摘要如今，伤口敷料作为高级皮肤产品，主要目的是加速伤口愈合过程。具有局部和延长释放治疗剂潜力的伤口敷料引起了极大的关注。在这项研究中，海藻酸盐/明胶 (AG) 混合水凝胶中负载的 niosomal 芦荟 (AV) 的持续释放的合成旨在改善作为伤口敷料的皮肤再生。为此，合成载有 AV 的 niosomes 并将其掺入混合 AG 水凝胶中。表征了 niosomes 的大小和多分散指数 (PdI)、AV 截留功效和 AV 体外释放。此外，还研究了水凝胶特性，如溶胀率、降解行为和机械性能。MTT 测定用于评估 AV 掺入和释放对成纤维细胞增殖的影响。结果表明，载有 AV 的 niosomes 的大小、PdI 和 EE% 分别为 270.080 nm、0.108 和 42.039 ± 4.090%，7 天后 AV 的体外释放约为 20%。由 niosomal AV 加载的 AG 混合水凝胶显示出延长缓释方式，其溶胀率和降解百分比在 72 小时后分别为 60 wt% 和 6 天后为 70 wt%。混合水凝胶的平均杨氏模量约为 12.64 ± 1.3 kPa，这似乎适合作为伤口敷料。最后，MTT 分析承认存在 AV 时成纤维细胞增殖增加，尤其是在载有 niosomal AV 的水凝胶中。最后，藻酸盐/凝胶混合水凝胶与 niosomal AV 结合，具有持续释放潜力可以被认为是伤口敷料应用的有希望的候选者。图形概要