There is compelling evidence in the literature to support the application of Raman spectroscopy for analysis of bodily fluids in their native liquid state. Naturally, the strategies described in the literature for Raman spectroscopic analysis of liquid samples have advantages and disadvantages. Herein, recent advances in the analysis of plasma/serum in the liquid state are reviewed. The potential advantages of Raman analysis in the liquid form over the commonly employed infrared absorption analysis in the dried droplet form are initially highlighted. Improvements in measurement protocols based on inverted microscopic geometries, clinically adaptable substrates, data preprocessing and analysis and applications for routine monitoring of patient health as well as therapeutic administration are reviewed. These advances suggest that clinical translation of Raman spectroscopy for rapid biochemical analysis can be a reality. In the future, this method will prove to be highly beneficial to clinicians for rapid screening and monitoring of analytes and drugs in the biological fluids, and to the patients themselves, enabling early treatment, before the disease becomes symptomatic, allowing early recovery.

中文翻译：

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拉曼光谱在分析液态血浆/血清中的潜力：最新进展。

文献中有令人信服的证据支持拉曼光谱法在天然液体体液分析中的应用。自然地，文献中描述的用于拉曼光谱分析液体样品的策略各有利弊。本文中，回顾了液态血浆/血清分析的最新进展。首先强调了液体形式的拉曼分析相对于干燥液滴形式的常用红外吸收分析的潜在优势。综述了基于倒置显微几何形状，临床适应性基质，数据预处理和分析的测量方案的改进以及对患者健康进行常规监测以及治疗性给药的应用。这些进展表明，用于快速生化分析的拉曼光谱的临床翻译可能是现实的。将来，这种方法将对临床医生快速筛查和监测生物体液中的分析物和药物，以及对患者本身非常有益，从而可以在疾病有症状之前及早治疗，从而早日康复。