Molybdenum disulfide quantum dots (MoS2 QDs) were chosen as a functional two-dimensional material to improve the separation performance of a traditional C18 column. In this work, MoS2 QDs were synthesized by the combination of sonication and solvothermal treatment of bulk MoS2. The prepared MoS2 QDs were characterized by transmission electron microscope (TEM), Zeta potential measurement, UV-visible absorption and fluorescence spectroscopy. Then, a novel MoS2 QDs embedded C18 (Sil-MoS2-C18) stationary phase was prepared for performing mixed-mode liquid chromatography. The results of elemental analysis (EA), thermogravimetric analysis (TGA), Fourier transform infrared spectrometry (FT-IR), X-ray photoelectron spectroscopy (XPS), scanning electron microscope (SEM) and Brunauer-Emmett-Teller (BET) measurements indicated the stationary phase was prepared successfully. Five types of compounds including alkylbenzenes, polycyclic aromatic hydrocarbons (PAHs), nucleosides and nucleobases, anilines and flavonoids were utilized to evaluate reversed phase, weak cation exchange and hydrophilic interaction of the new column. To a certain extent, the column could achieve separation for different properties of samples on one column, with less organic solvent and shorter time than conventional alkyl and amino columns. Furthermore, the mechanism for separation was studied by investigating effects of mobile phase composition and pH on retentions. In summary, the Sil-MoS2-C18 stationary phase was deemed able to serve the performance of various types of phases, which revealed the prepared mixed-mode column could be potentially applied for the analysis of complex samples. Graphical abstract.

中文翻译：

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嵌入C18混合模式色谱固定相的二硫化钼量子点的制备和评估。

选择二硫化钼量子点（MoS2 QDs）作为功能性二维材料，以改善传统C18色谱柱的分离性能。在这项工作中，MoS2量子点是通过超声处理和溶剂热处理对大量MoS2进行合成的。通过透射电子显微镜（TEM），Zeta电位测量，紫外可见吸收和荧光光谱对制备的MoS2量子点进行了表征。然后，制备了新型的嵌入MoS2 QDs的C18（Sil-MoS2-C18）固定相，以进行混合模式液相色谱分析。元素分析（EA），热重分析（TGA），傅立叶变换红外光谱（FT-IR），X射线光电子能谱（XPS），扫描电子显微镜（SEM）和Brunauer-Emmett-Teller（BET）测量表明固定相已成功制备。利用五种类型的化合物，包括烷基苯，多环芳烃（PAH），核苷和核碱基，苯胺和类黄酮，评估了新色谱柱的反相，弱阳离子交换和亲水相互作用。在一定程度上，该色谱柱可以在一根色谱柱上实现样品不同性质的分离，与传统的烷基和氨基色谱柱相比，有机溶剂更少，时间更短。此外，通过研究流动相组成和pH对保留的影响，研究了分离机理。总之，Sil-MoS2-C18固定相被认为能够满足各种类型的相的需要，这表明制备的混合模式色谱柱可潜在地用于复杂样品的分析。图形概要。