Neurodegeneration currently remains without a differential diagnosis or cure. Tau protein is one of the biomarkers of neurodegenerative diseases commonly known as tauopathies. Tau protein plays an integral role in stabilizing microtubules and cell structure; however, due to post-translational modifications, tau protein undergoes self-assembly into cytotoxic structures and is co-localized intra- and extracellularly. Hence, tau protein is a viable biomarker associated with protein pathogenesis and neurodegeneration. The novel optical biosensor for tau441 protein is based on the aptamer recognition probe and the biolayer interferometry (BLI) method for detection. The current biotin-aptasensor in combination with the streptavidin surface provides real-time monitoring of tau441 protein in the nanomolar range, with the limit of detection at 6.7 nM in vitro. The tau441 detection is achieved with high selectivity over other neurodegeneration biomarkers which include amyloid-β and α-synuclein. The aptasensor also allows for tau441 protein detection in a complex matrix such as fetal bovine serum, indicating its utility in other biological fluids for diagnostic applications. The optical method is simple, rapid and highly selective for point-of-care application which is critical for achieving the early and differential diagnosis of neurodegenerative diseases and identifying their treatments. Graphical abstract.

中文翻译：

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一种用于读取tau蛋白的即读即读光学适体传感器。

目前，神经变性仍然没有鉴别诊断或治愈。Tau蛋白是神经退行性疾病的生物标记物之一，通常被称为tauopathies。Tau蛋白在稳定微管和细胞结构中起着不可或缺的作用。然而，由于翻译后修饰，tau蛋白自组装成细胞毒性结构，并在细胞内和细胞外共定位。因此，tau蛋白是与蛋白发病机理和神经变性相关的可行生物标志物。用于tau441蛋白的新型光学生物传感器基于适体识别探针和生物层干涉术（BLI）方法进行检测。当前的生物素-适体传感器与抗生蛋白链菌素表面结合可实时监测纳摩尔浓度范围内的tau441蛋白，检测极限为6。体外7 nM。相对于其他神经变性生物标记物（包括淀粉样蛋白-β和α-突触核蛋白），tau441检测具有高选择性。适体传感器还允许在复杂的基质（如胎牛血清）中检测tau441蛋白，从而表明其可用于其他生物流体进行诊断。光学方法简单，快速且高度选择性，适用于即时护理，这对于实现神经退行性疾病的早期和鉴别诊断并确定其治疗方法至关重要。图形概要。表明其在其他生物液体中用于诊断应用的效用。光学方法简单，快速且高度选择性，适用于即时护理，这对于实现神经退行性疾病的早期和鉴别诊断并确定其治疗方法至关重要。图形概要。表明其在其他生物液体中用于诊断应用的效用。光学方法简单，快速且高度选择性，适用于即时护理，这对于实现神经退行性疾病的早期和鉴别诊断并确定其治疗方法至关重要。图形概要。