In recent years, the research on stem cells has been more and more in-depth, and many achievements have been made in application. However, due to the small number of spermatogonial stem cells (SSCs) and deficiency of efficient markers, it is difficult to obtain very pure SSCs, which results in the research on them being hindered. In fact, many methods have been developed to isolate and purify SSCs, but these methods have their shortcomings. Fluorescence-activated cell sorting (FACS), as a method to enrich SSCs with the help of specific surface markers, has the characteristics of high efficiency and accuracy in enrichment of SSCs, thus it is widely accepted as an effective method for purification of SSCs. This review summarizes the recent studies on the application of FACS in SSCs, and introduces some commonly used markers of effective SSCs sorting, aiming to further optimize the FACS sorting method for SSCs, so as to promote the research of germline stem cells and provide new ideas for the research of reproductive biology.

中文翻译：

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精原干细胞利用荧光激活细胞分选技术的研究进展。

近年来，对干细胞的研究越来越深入，在应用方面取得了许多成就。然而，由于精原干细胞（SSCs）数量少和有效标记物的缺乏，很难获得非常纯的SSC，这阻碍了对它们的研究。实际上，已经开发了许多分离和纯化SSC的方法，但是这些方法都有其缺点。荧光激活细胞分选术（FACS）作为一种借助特定表面标记物富集SSC的方法，具有高效，准确富集SSC的特性，因此被广泛认为是一种纯化SSC的有效方法。这篇综述总结了关于FACS在SSC中的应用的最新研究，并介绍了一些有效的SSC分类的常用标记，