The frequency of congenital malformations is 3-5 times higher in mothers with pregestational diabetes mellitus than in general population. Apparently, this problem is due to change in the expression of apoptotic and antiapoptotic genes induced by the oxidative stress derived from the diabetes/hyperglycemia. One of these genes is Bcl-2, which is associated with the control and inhibition of apoptosis. The purpose of the present work was to study the effect of polyamine addition over expression of Bcl-2 gene in a model of diabetic embryopathy. For this, gestational day 10.5 (GD10.5) rat embryos were incubated at 37°C for 24 h in control medium, medium with high glucose, or medium with high glucose and supplemented with spermidine or spermine. Post-cultured embryos were harvested and observed to obtain morphological scores; some of them were subjected to molecular biology studies: DNA isolation plus conventional PCR or RNA isolation plus RT-PCR; other embryos were fixed with paraformaldehyde and used for immunohistochemical detection of Bcl-2 protein. Although Bcl-2 mRNA was similarly expressed in all rat embryo treatments, Bcl-2 protein was found only in control-incubated embryos. In conclusion, it seems that the inhibition of Bcl-2 gene expression induced by glucose was not reversed by polyamines.

中文翻译：

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在多胺存在下的糖尿病胚胎病变模型中Bcl-2表达。

患有妊娠糖尿病的母亲的先天性畸形的发生率是普通人群的3-5倍。显然，该问题归因于由糖尿病/高血糖症引起的氧化应激诱导的凋亡和抗凋亡基因表达的变化。这些基因之一是Bcl-2，它与细胞凋亡的控制和抑制有关。本工作的目的是研究在糖尿病胚胎病模型中多胺添加对Bcl-2基因表达的影响。为此，将妊娠第10.5天（GD10.5）的大鼠胚胎在37°C下于对照培养基，高葡萄糖培养基或高葡萄糖培养基中添加亚精胺或亚精胺培养24小时。收获培养后的胚胎并观察以获得形态学评分；其中一些进行了分子生物学研究：DNA分离加常规PCR或RNA分离加RT-PCR；其他胚胎用低聚甲醛固定并用于Bcl-2蛋白的免疫组化检测。尽管Bcl-2 mRNA在所有大鼠胚胎处理中均类似表达，但Bcl-2蛋白仅在对照培养的胚胎中发现。总之，看来多胺不能逆转葡萄糖诱导的对Bcl-2基因表达的抑制作用。