In this study, we propose a microchip that is sequentially capable of fluorescently staining and washing DNAs. The main advantage of this microchip is that it allows for one-step preparation of small amounts of solution without degrading microscopic bio-objects such as the DNAs, cells, and biomolecules to be stained. The microchip consists of two inlets, the main channel, staining zone, washing zone, and one outlet, and was processed using a femtosecond laser system. High molecular transport of rhodamine B to deionized water was observed in the performance test of the microchip. Results revealed that the one-step procedure of on-chip DNA staining and washing was excellent compared to the conventional staining method. The one-step preparation of stained and washed DNAs through the microchip will be useful for preparing small volumes of experimental samples.

中文翻译：

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用于DNA荧光标记的一步式微芯片。

在这项研究中，我们提出了一种微芯片，该芯片能够依次进行荧光染色和洗涤DNA。这种微芯片的主要优点在于，它可以一步制备少量溶液，而不会降解微观生物物体，例如要染色的DNA，细胞和生物分子。微芯片由两个入口组成，分别是主通道，染色区，洗涤区和一个出口，并使用飞秒激光系统进行了处理。在微芯片的性能测试中，观察到若丹明B向去离子水中的高分子迁移。结果表明，与常规染色方法相比，片上DNA染色和洗涤的一步操作非常出色。