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One-step microchip for DNA fluorescent labeling

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Abstract

In this study, we propose a microchip that is sequentially capable of fluorescently staining and washing DNAs. The main advantage of this microchip is that it allows for one-step preparation of small amounts of solution without degrading microscopic bio-objects such as the DNAs, cells, and biomolecules to be stained. The microchip consists of two inlets, the main channel, staining zone, washing zone, and one outlet, and was processed using a femtosecond laser system. High molecular transport of rhodamine B to deionized water was observed in the performance test of the microchip. Results revealed that the one-step procedure of on-chip DNA staining and washing was excellent compared to the conventional staining method. The one-step preparation of stained and washed DNAs through the microchip will be useful for preparing small volumes of experimental samples.

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Acknowledgments

This work was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2017R1A4A1015681, NRF-2018R1D1A3B07047434, and 2019R1I1A3A01060695).

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Author notes

  1. Yeongseok Jang and Hojun Shin equally contributed to this article.

Authors and Affiliations

  1. Department of Mechanical Design Engineering, College of Engineering, Chonbuk National University, Jeonju, 54896, South Korea

    Yeongseok Jang

  2. Advanced Development Group, Samsung SDI Co., Yongin, 17084, South Korea

    Hojun Shin

  3. Department of Nano-Bio Mechanical System Engineering, College of Engineering, Chonbuk National University, Jeonju, 54896, South Korea

    Jinmu Jung & Jonghyun Oh

Authors
  1. Yeongseok Jang
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  2. Hojun Shin
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  3. Jinmu Jung
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  4. Jonghyun Oh
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Contributions

Conceptualization, Jinmu Jung and Jonghyun Oh; Data curation, Yeongseok Jang and Hojun Shin; Formal analysis, Yeongseok Jang and Hojun Shin; Investigation, Yeongseok Jang and Hojun Shin; Project administration, Jinmu Jung and Jonghyun Oh; Supervision, Jinmu Jung and Jonghyun Oh; Writing—original draft, Yeongseok Jang and Hojun Shin; Writing—review & editing, Jinmu Jung and Jonghyun Oh.

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Correspondence to Jinmu Jung or Jonghyun Oh.

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Jang, Y., Shin, H., Jung, J. et al. One-step microchip for DNA fluorescent labeling. Biomed Microdevices 22, 1 (2020). https://doi.org/10.1007/s10544-019-0454-1

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