Mutanases are enzymes that have the ability to cleave α-1,3 linkages in glucan polymer. In the present investigation, mutanase enzyme purified from the culture filtrate of Paracoccus mutanolyticus was evaluated for Streptococcal biofilm degradation and antimicrobial activity against pathogenic fungi along with enzyme kinetics, activation energies, pH and thermal stability. Biochemical and molecular characterization depicted that the enzyme showed optimum activity at pH 5.5 and at 50 °C. It displayed Michaelis–Menten behaviour with a K_m of 1.263 ± 0.03 (mg/ml), V_max of 2.712 ± 0.15 U/mg protein. Thermal stability studies denoted that it required 55.46 and 135.43 kJ mol⁻¹ of energy for activation and deactivation in the temperature range of 30–50 °C and 50–70 °C respectively. Mutanase activity was enhanced ~ 50 and 75% by Fe²⁺ and EDTA, respectively, while presence of Hg²⁺ and Mn²⁺ inhibit > 90% of its activity. This enzyme has a molecular mass of 138 kDa and showed monomeric nature by Zymography. Scanning electron microscopy analysis of mutanase treated Streptococcal cells revealed cleavage of linkages among the cells and complete separation of cells, indicating its potential in dentistry as an anticaries agent in the prophylaxis and therapy of dental caries. In addition, antifungal activity of mutanase against Colletotrichum capsici MTCC 10147 and Cladosporium cladosporioide MTCC 7371 revealed that the enzyme has potential towards biological control of phytopathogens which could be used as an alternative bio-control agent against chemical pesticides in the future.

中文翻译：

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来自变形副球菌RSP02的变形酶：表征和作为生物防治剂的应用。

突变酶是具有裂解葡聚糖聚合物中α-1,3键的能力的酶。在本研究中，评估了从变形副球菌培养滤液中纯化的诱变酶的链球菌生物膜降解和对病原真菌的抗菌活性，以及​​酶动力学，活化能，pH和热稳定性。生化和分子表征表明该酶在pH 5.5和50°C下显示最佳活性。它以1.263±0.03（mg / ml）的K _m表现出Michaelis–Menten行为，V _max为2.712±0.15 U / mg蛋白质。热稳定性研究表明它需要55.46和135.43 kJ mol ^-1分别在30–50°C和50–70°C的温度范围内用于激活和停用的能量。Fe ²⁺和EDTA分别将突变酶活性提高了约50％和75％，而Hg ²⁺和Mn ^2+的存在抑制了90％以上的活性。该酶的分子量为138kDa，并通过Zymography显示出单体性质。诱变酶处理的链球菌细胞的扫描电子显微镜分析揭示了细胞间连接的裂解和细胞的完全分离，表明其在牙科中作为防龋剂在预防和治疗龋齿中的潜力。此外，诱变酶对辣椒炭疽菌MTCC 10147和Cladosporium cladosporioide MTCC 7371揭示了该酶具有对植物病原体进行生物控制的潜力，将来可以用作化学农药的替代生物控制剂。