BRD7 is a potential nuclear transcription regulation factor related to nasopharyngeal carcinoma (NPC). BRD2, a putative BRD7-interacting protein, has been screened from human fetal brain cDNA library by yeast two-hybrid system. This study was to further identify the interaction between BRD7 and BRD2 in mammalian cells, and to investigate the subcellular localization of BRD2, as well as the effect on the functions of cell biology. Both immunoprecipitation and subcellular colocalization were performed together to identify the interaction of BRD7 with full-length BRD2, as well as C-terminal truncated BRD2 or N-terminal truncated BRD2. GFP direct fluorescence and Hochest 33258 staining were used to investigate the cellular localization pattern of BRD2 and the roles in initiating cell apoptosis in COS7 and HNE1. The results showed that BRD7 could interact with BRD2 and the region from amino acid 430 to 798 of BRD2 was critical for the interaction of BRD2 with BRD7. BRD2 mainly localizes in nucleus in two distribution patterns, diffused and dotted, and BRD2 has distinct roles in initiating apoptosis, and the dotted distribution pattern of BRD2 in nucleus may be a morphologic marker of cell apoptosis.

中文翻译：

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BRD2是与BRD7相互作用的蛋白之一，其过表达可引发细胞凋亡。

BRD7是与鼻咽癌（NPC）相关的潜在核转录调控因子。BRD2是一种假定的BRD7相互作用蛋白，已通过酵母双杂交系统从人胎脑cDNA文库中筛选出来。这项研究旨在进一步鉴定哺乳动物细胞中BRD7和BRD2之间的相互作用，并研究BRD2的亚细胞定位以及对细胞生物学功能的影响。一起进行了免疫沉淀和亚细胞共定位，以鉴定BRD7与全长BRD2以及C端截短的BRD2或N端截短的BRD2的相互作用。使用GFP直接荧光和Hochest 33258染色研究BRD2的细胞定位模式以及在COS7和HNE1中启动细胞凋亡的作用。结果表明，BRD7可以与BRD2相互作用，并且BRD2的430-798位氨基酸对于BRD2与BRD7的相互作用至关重要。BRD2主要分布在细胞核中，呈散布和点状两种分布模式，BRD2在细胞凋亡的启动中起不同的作用，BRD2在细胞核中的散布分布模式可能是细胞凋亡的形态学标志。