The intensity of erythrocyte membrane fluctuations was studied by laser interference microscopy (LIM), which provide information about mechanical properties of the erythrocyte membrane. Atomic force microscopy (AFM) was used to study erythrocyte surface relief; it is related to the cytoskeleton structure of erythrocyte membrane. Intact human erythrocytes and erythrocytes with a destroyed cytoskeleton were used. According to the obtained results, cytoskeleton damage induced by heating up to 50 °С results in a reduced intensity of cell membrane fluctuations compared to non-treated cells (20.6 ± 10.2 vs. 30.5 ± 5.5 nm, correspondingly), while the roughness of the membrane increases (4.5 ± 1.5 vs. 3.4 ± 0.5 nm, correspondingly).

中文翻译：

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红细胞中细胞骨架的热损伤增加膜粗糙度和细胞刚性

通过激光干涉显微镜 (LIM) 研究红细胞膜波动的强度，它提供了有关红细胞膜机械性能的信息。原子力显微镜 (AFM) 用于研究红细胞表面起伏；它与红细胞膜的细胞骨架结构有关。使用完整的人红细胞和细胞骨架被破坏的红细胞。根据获得的结果，与未处理的细胞相比，由加热至 50 °C 引起的细胞骨架损伤导致细胞膜波动强度降低（20.6 ± 10.2 vs. 30.5 ± 5.5 nm，相应地），而粗糙度膜增加（4.5 ± 1.5 与 3.4 ± 0.5 nm，相应地）。