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Interactions of Cinnamycin-Immobilized Gold Nanorods with Biomimetic Membranes.
The Journal of Membrane Biology ( IF 2.3 ) Pub Date : 2019-11-22 , DOI: 10.1007/s00232-019-00103-3
Gil Sun Lee 1 , Jin-Won Park 2
Affiliation  

The behavior of the cinnamycin immobilized on the gold nanorod(AuNR) was investigated using surface plasmon resonance(SPR). For the comparison of the immobilized cinnamycin, the study for the free cinnamycin was also conducted. The bilayer was fabricated by tethering 1,2-dipalmitoyl-sn-glycero-3-phosphothioethanols on a gold surface to form a monolayer and then using liposomes to adsorb an outer layer on the tethered-monolayer. The liposomes were prepared with a desired ratio of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine to 1,2-dioleoyl-sn-glycero-3-phosphocholine (0:100, 5:95, 10:90, 20:80, and 30:70). After the cinnamycin was injected on the bilayers, the specific binding between the cinnamycin and the bilayer was monitored with SPR. The inclusion of DOPE in the outer layer clearly led to the specific binding of the cinnamycin on the membranes. Specifically, the binding behavior of the immobilized was different from that of the free. For the free cinnamycin, the binding amount of cinnamycin at 10% was two times more than that at 5%. For the immobilized cinnamycin, the amounts were identical for both compositions. However, the rate was much faster for the immobilized cinnamycin at 10% than 5%, compared to that for the free at both compositions. This difference was attributed to the mean-molecular areas of the cinnamycin and DOPE, and the steric effect of the AuNR. For the effects of the heat and storage, the immobilized enzyme showed less decrease in the relative binding amount than the free one.

中文翻译:

固定有肉桂霉素的金纳米棒与仿生膜的相互作用。

利用表面等离子体共振(SPR)研究了固定在金纳米棒(AuNR)上的肉桂霉素的行为。为了比较固定的肉桂霉素,还进行了游离肉桂霉素的研究。通过在金表面上束缚1,2-二棕榈酰基-sn-甘油-3-磷酸硫代乙醇以形成单层,然后使用脂质体将外层吸附在束缚的单层上来制造双层。制备脂质体,使其具有所需比例的1,2-二油酰基-sn-甘油-3-磷酸乙醇胺与1,2-二油酰基-sn-甘油-3-磷酸胆碱(0:100、5:95、10:90、20 :80和30:70)。在将肉桂霉素注射到双层上之后,用SPR监测肉桂霉素和双层之间的特异性结合。外层中包含DOPE明显导致了肉桂霉素在膜上的特异性结合。具体地,固定化的结合行为不同于游离的结合行为。对于游离的肉桂霉素,肉桂霉素的结合量在10%时是在5%时的两倍。对于固定的肉桂霉素,两种组合物的量相同。然而,与两种组合物中游离的肉桂霉素相比,固定的肉桂霉素的转化率分别为10%比5%快得多。这种差异归因于肉桂霉素和DOPE的平均分子面积以及AuNR的空间效应。就热量和储存的影响而言,固定化酶的相对结合量降低的程度比游离酶低。固定化的结合行为不同于游离的。对于游离的肉桂霉素,肉桂霉素的结合量在10%时是在5%时的两倍。对于固定的肉桂霉素,两种组合物的量相同。然而,与两种组合物中游离的肉桂霉素相比,固定的肉桂霉素的转化率分别为10%比5%快得多。这种差异归因于肉桂霉素和DOPE的平均分子面积以及AuNR的空间效应。就热量和储存的影响而言,固定化酶的相对结合量降低的程度比游离酶低。固定化的结合行为不同于游离的。对于游离的肉桂霉素,肉桂霉素的结合量在10%时是在5%时的两倍。对于固定的肉桂霉素,两种组合物的量相同。然而,与两种组合物中游离的肉桂霉素相比,固定的肉桂霉素的转化率分别为10%比5%快得多。这种差异归因于肉桂霉素和DOPE的平均分子面积以及AuNR的空间效应。就热量和储存的影响而言,固定化酶的相对结合量减少量少于游离酶。与两种组合物中游离的辛那霉素相比,固定的辛那霉素(分别为10%和5%)的转化率要快得多。这种差异归因于肉桂霉素和DOPE的平均分子面积以及AuNR的空间效应。就热量和储存的影响而言,固定化酶的相对结合量减少量少于游离酶。与两种组合物中游离的辛那霉素相比,固定的辛那霉素(分别为10%和5%)的转化率要快得多。这种差异归因于肉桂霉素和DOPE的平均分子面积以及AuNR的空间效应。就热量和储存的影响而言,固定化酶的相对结合量降低的程度比游离酶低。
更新日期:2020-04-14
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