Salivirus is a newly discovered virus which seems to be related to acute gastroenteritis in children. Salivirus may infect susceptible children by fecal–oral route after exposure to contaminated water. The present study aims to evaluate the occurrence and quantity of Salivirus in treated and untreated sewage water and river water samples collected in the city of Karaj, Iran by reverse transcription-quantitative PCR assay. A total of 50 samples were collected from environmental waters containing 22 treated and untreated sewage water in volume of 1 l and 28 river water samples in volume of 5 l were included in this study. After viral RNA extraction, the Real-time PCR was performed to amplify the 5′UTR sequence of Salivirus genome and viral load was assessed. Out of the 50 samples tested, the Salivirus genomic RNA was identified in 5/12 (41.6%) of treated and 3/10 (30%) of untreated sewage samples and in 8/28 (28.5%) of river water samples. The maximum viral load was 4.8 × 10⁶ copies/l in treated sewage water sample in September and the lower viral load was 4 × 10⁵ copies/l related to treated sewage water taken in December. This is the first report of Salivirus occurrence in the environmental waters in Iran. The viral prevalence of Salivirus in each of the three sets of tested samples was within low to moderate in range.

中文翻译：

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伊朗卡拉伊的污水和河水样本中存在唾液病毒。

唾液病毒是一种新发现的病毒，似乎与儿童急性胃肠炎有关。唾液病毒暴露于受污染的水后，可能通过粪便途径感染易感儿童。本研究旨在通过逆转录定量PCR分析评估在伊朗卡拉伊市收集的处理过的和未经处理的污水和河水样品中的唾液病毒的发生和数量。从环境水中总共收集了50个样品，其中包含22份1l体积的处理过的和未处理的污水，以及28份5l体积的河水样品。病毒RNA提取后，进行实时PCR扩增唾液病毒基因组的5'UTR序列，并评估病毒载量。在测试的50个样本中，Salivirus基因组RNA的鉴定率为5/12（41。经处理的污水样本占6％），未经处理的污水样本占3/10（30％），在河水样本中占8/28（28.5％）。最大病毒载量为4.8×10^6个拷贝/升处理过的污水样品中九月和下病毒载量为4×10 ^5个与在十二月采取处理过的污水水拷贝/升。这是首次在伊朗环境水域出现唾液病毒的报道。在三组测试样品中的每组中，唾液病毒的病毒流行率均在低至中等范围内。