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Detection of mPing mobilization in transgenic rice plants.
Genes & Genomics ( IF 1.6 ) Pub Date : 2019-11-12 , DOI: 10.1007/s13258-019-00877-9
Doori Park 1 , Ik-Young Choi 2 , Nam-Soo Kim 1
Affiliation  

BACKGROUND Various kinds of transposable elements (TEs) constitute high proportions of eukaryotic genomes. Although most of these TEs are not actively mobile, genome stress can induce mobilization of dormant TEs. Transgenic plants undergo tissue culture and subsequent whole-plant regeneration, which can cause genomic stress and in turn induce mobilization of inactive TEs. OBJECTIVES To investigate the activation of transposable elements on the genome wide of the GM plant. METHODS Transposon activities were analyzed in three transgenic rice plants carrying the insect resistance gene Cry1Ac and an herbicide resistance gene by the transposon display technique. These three transgenic plants were derived from a leading Korean rice variety, Illmi. RESULTS We detected seven mobile activities in the mPing element, which is a MITE family transposon. The identity of the novel fragments in the gel display was confirmed by checking TAA target site duplication via sequence analysis. The genomic integration sites were all on different chromosomes, and the integrations were specific to either one or two T1 transgenic lines, except for one common integration on chromosome 4. One integration was in the 5'-UTR of the Glycerol-3-phosphate acyltransferase 8 gene, two integrations were in introns of expressed genes, and the other four integrations were in intergenic regions. CONCLUSION Thus, novel mobilization of dormant TEs occurs in transgenic plants, which must be considered in the generation of genetically modified crops (GM crops).

中文翻译:

转基因水稻植株中mPing动员的检测。

背景技术各种可转座元件(TEs)构成高比例的真核基因组。尽管大多数这些TE不能主动移动,但是基因组压力可以诱导休眠TE的动员。转基因植物经过组织培养和随后的全植物再生,这可能引起基因组压力,进而诱导非活性TE的动员。目的研究转基因元件在转基因植物全基因组中的激活。方法通过转座子展示技术,分析了三种具有昆虫抗性基因Cry1Ac和除草剂抗性基因的转基因水稻植株的转座子活性。这三种转基因植物均来自韩国领先的水稻品种Illmi。结果我们在mPing元件(一种MITE家族转座子)中检测到七个移动活动。通过序列分析检查TAA靶位点重复,证实了凝胶展示中新片段的身份。基因组整合位点都在不同的染色体上,整合对一个或两个T1转基因系都具有特异性,除了在染色体4上的一个常见整合。一个整合是甘油3-磷酸酰基转移酶的5'-UTR。 8个基因中,两个整合位于表达基因的内含子中,其他四个整合位于基因间区域。结论因此,休眠的TE的新动员发生在转基因植物中,这在转基因作物(转基因作物)的产生中必须考虑。基因组整合位点都在不同的染色体上,整合对一个或两个T1转基因系都具有特异性,除了在染色体4上的一个常见整合。一个整合是甘油3-磷酸酰基转移酶的5'-UTR。 8个基因中,两个整合位于表达基因的内含子中,其他四个整合位于基因间区域。结论因此,休眠的TE的新动员发生在转基因植物中,这在转基因作物(转基因作物)的产生中必须考虑。基因组整合位点都在不同的染色体上,整合对一个或两个T1转基因系都具有特异性,除了在染色体4上的一个常见整合。一个整合是甘油3-磷酸酰基转移酶的5'-UTR。在8个基因中,两个整合位于表达基因的内含子中,其他四个整合位于基因间区域。结论因此,休眠的TE的新动员发生在转基因植物中,这在转基因作物(转基因作物)的产生中必须考虑。其他四个整合位于基因间区域。结论因此,休眠的TE的新动员发生在转基因植物中,这在转基因作物(转基因作物)的产生中必须考虑。其他四个整合位于基因间区域。结论因此,休眠的TE的新动员发生在转基因植物中,这在转基因作物(转基因作物)的产生中必须考虑。
更新日期:2019-11-01
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