Abstract Pseudorabies virus (PRV) UL2 (pUL2) is a multifunctional protein, which is homologous with herpes simplex virus 1 early protein UL2 (hUL2) and crucial for the viral propagation. Yet, how pUL2 executes its roles in the viral life cycle remain inadequately understood. In order to uncover its effect on the procedure of PRV infection, investigation was performed to examine the subcellular distribution of pUL2 and establish its trafficking mechanism. In the present study, enhanced yellow fluorescent protein or Myc tag fused pUL2 was transiently overexpressed in transfected cells and exhibited an absolutely nuclear accumulation without the existence of other PRV proteins. Additionally, the nuclear trafficking of pUL2 was proved to rely on Ran-, transportin-1, importin β1, importin α1, α3 and α5. Accordingly, these data will benefit the knowledge of pUL2-mediated biological effects in PRV infection cycle.

中文翻译：

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伪狂犬病病毒UL2细胞内分布及其核输入机制检测

摘要 伪狂犬病病毒（PRV）UL2（pUL2）是一种多功能蛋白，与单纯疱疹病毒1早期蛋白UL2（hUL2）同源，对病毒的繁殖至关重要。然而，pUL2 如何在病毒生命周期中发挥作用仍然没有得到充分了解。为了揭示其对 PRV 感染过程的影响，进行了调查以检查 pUL2 的亚细胞分布并建立其运输机制。在本研究中，增强的黄色荧光蛋白或 Myc 标记融合的 pUL2 在转染细胞中瞬时过表达，并且在没有其他 PRV 蛋白存在的情况下表现出绝对的核积累。此外，pUL2 的核运输被证明依赖于 Ran-、transportin-1、importin β1、importin α1、α3 和 α5。因此，