Abstract
Pseudorabies virus (PRV) UL2 (pUL2) is a multifunctional protein, which is homologous with herpes simplex virus 1 early protein UL2 (hUL2) and crucial for the viral propagation. Yet, how pUL2 executes its roles in the viral life cycle remain inadequately understood. In order to uncover its effect on the procedure of PRV infection, investigation was performed to examine the subcellular distribution of pUL2 and establish its trafficking mechanism. In the present study, enhanced yellow fluorescent protein or Myc tag fused pUL2 was transiently overexpressed in transfected cells and exhibited an absolutely nuclear accumulation without the existence of other PRV proteins. Additionally, the nuclear trafficking of pUL2 was proved to rely on Ran-, transportin-1, importin β1, importin α1, α3 and α5. Accordingly, these data will benefit the knowledge of pUL2-mediated biological effects in PRV infection cycle.
Funding source: National Natural Science Foundation of China
Award Identifier / Grant number: 81772179
Funding source: Natural Science Foundation of Guangdong Province
Award Identifier / Grant number: 2018A0303130257
Award Identifier / Grant number: 2015A030313473
Award Identifier / Grant number: 2019A1515010395
Funding source: Education Department of Guangdong Province, China
Award Identifier / Grant number: 2018KTSCX184
Funding statement: This work was supported by grants from the National Natural Science Foundation of China (Funder Id: http://dx.doi.org/10.13039/501100001809, 81772179); the Natural Science Foundation of Guangdong Province (2018A0303130257, 2015A030313473 and 2019A1515010395); the Regular University Distinguished Innovation Project from Education Department of Guangdong Province, China (2018KTSCX184); the Medical Scientific Research Foundation of Guangdong Province, China (A2017055); High-Level Universities Academic Backbone Development Program of Guangzhou Medical University; and the Undergraduate Laboratory Opening Project of Guangzhou Medical University (2018 and 2019).
Competing interests: The authors declare that they have no competing interests.
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