Many natural products could induce apoptosis through mitochondrial pathways. However, direct interactions between natural products and mitochondria have rarely been reported. In this work, the effects and regulatory mechanisms of Jaceosidin on the isolated rat liver mitochondria have been studied. The results of the experiments which by introducing exogenous Ca2+ illustrated that Jaceosidin has the protective effects on the structure and function of the isolated mitochondria. These protective effects were related to the chelation of Ca2+ with Jaceosidin. Besides, Jaceosidin could scavenge reactive oxygen species produced during electron transport, and weaken the mitochondrial lipid peroxidation rate, which may be attributed to the antioxidant effect of phenolic hydroxyl groups of Jaceosidin. In addition, Jaceosidin has some damage effects on mitochondrial function, such as the inhibition of mitochondrial respiration and the increase of mitochondrial membrane fluidity. These results of this work provided comprehensive information to clarify the mechanisms of Jaceosidin on mitochondria, which may be the bidirectional regulatory mechanisms.

中文翻译：

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鞘脂对线粒体功能的双向调节机制：渗透性转变和膜功能损害的保护作用。

许多天然产物可以通过线粒体途径诱导细胞凋亡。但是，很少有天然产物和线粒体之间直接相互作用的报道。在这项工作中，研究了菊苣素对离体大鼠肝脏线粒体的作用和调节机制。通过引入外源Ca 2+的实验结果表明，鞘脂苷对分离的线粒体的结构和功能具有保护作用。这些保护作用与Jaceosidin与Ca2 +的螯合有关。此外，Jaceosidin还可以清除电子传输过程中产生的活性氧，并减弱线粒体脂质的过氧化速率，这可能归因于Jaceosidin酚羟基的抗氧化作用。此外，菊苣苷对线粒体功能有一定的破坏作用，如抑制线粒体呼吸和增加线粒体膜流动性。这项工作的这些结果提供了全面的信息，阐明了鞘氨醇对线粒体的机制，这可能是双向调控机制。