Phage study draws more attention recently as the bacterial antibiotic resistances become a major threat for global health. Bacteriophage T4 is one of the most studied the phages and the representative of Tevenvirinae subfamily. Since 1950s, T4 phage has been studied more intensively than any other large lytic phages and its biological studies have provided basis for current phage biology as well as other applications. However, among approximately 300 T4 genes, 130 of them still remain uncharacterized. Coded by y04L gene in pin-nrdC intergenic region, Y04L is an example of such proteins whose biological function and mechanism are yet to be addressed. While Pin blocks bacterial Lon protease and thus inhibits bacterial toxin–antitoxin system, NrdC or Glutaredoxin is a specific reducing agent for the phage-induced ribonucleotide reductase. With two interesting neighbouring genes, this 11.9 kDa protein may be functionally related to Pin or NrdC. Here, using solution-state NMR, our near-complete resonance assignment of Y04L provides a basis for future structure determination and further mechanism study.

中文翻译：

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噬菌体T4 Y04L蛋白的共振分配。

随着细菌对抗生素的耐药性成为全球健康的主要威胁，噬菌体研究近来引起了更多关注。噬菌体T4是研究最多的噬菌体之一，是Tevenvirinae亚家族的代表。自1950年代以来，对T4噬菌体的研究比其他任何大型裂解噬菌体都更为深入，其生物学研究为当前的噬菌体生物学及其他应用提供了基础。但是，在大约300个T4基因中，其中130个仍未表征。Y04L由pin-nrdC基因间区域中的y04L基因编码，是此类蛋白质的一个例子，其生物学功能和机制尚待解决。当Pin阻断细菌Lon蛋白酶从而抑制细菌毒素-抗毒素系统时，NrdC或Glutaredoxin是噬菌体诱导的核糖核苷酸还原酶的特异性还原剂。具有两个有趣的相邻基因，此11.9 kDa蛋白可能与Pin或NrdC在功能上相关。在这里，使用溶液态NMR，我们对Y04L的接近完全共振分配为将来的结构确定和进一步的机理研究提供了基础。