Concentrations of the single-chain polypeptide hormone prolactin (PRL) are associated with wool or cashmere traits, and its seasonal changes may determine patterns of enzymatic activity and may affect cashmere fibre growth and moult. So, thePRL gene is a potential candidate gene for cashmere traits in marker-assisted selection (MAS). In this paper, we report a novel missense single-nucleotide polymorphism (SNP) within the goatPRL gene in 1367 individuals by PCR-SSCP (polymerase chain reaction with single-strand conformation polymorphism) analysis and DNA sequencing. The novel X76049:g.576C>A mutation is confirmed byEco24I PCR-RFLP (restriction fragment length polymorphism) analysis and causes a missense codon (Pro176Thr). The frequencies of allele C varied from 0.79 to 0.93 in 9 analysed goat populations. C allele was correlated with higher fibre length (P = 0.014).

中文翻译：

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与羊绒性状相关的山羊催乳素基因 (PRL) 的新 SNP。

单链多肽激素催乳素 (PRL) 的浓度与羊毛或羊绒特性有关，其季节性变化可能决定酶活性的模式，并可能影响羊绒纤维的生长和换羽。因此，PRL基因是标记辅助选择（MAS）中羊绒性状的潜在候选基因。在本文中，我们通过 PCR-SSCP（具有单链构象多态性的聚合酶链反应）分析和 DNA 测序报告了1367 只山羊PRL基因内的一种新型错义单核苷酸多态性 (SNP) 。新型 X76049:g.576C>A 突变被Eco证实24I PCR-RFLP（限制性片段长度多态性）分析并导致错义密码子 (Pro176Thr)。在 9 个分析的山羊种群中，等位基因 C 的频率从 0.79 到 0.93 不等。C 等位基因与较高的纤维长度相关（P = 0.014）。