Abstract
Concentrations of the single-chain polypeptide hormone prolactin (PRL) are associated with wool or cashmere traits, and its seasonal changes may determine patterns of enzymatic activity and may affect cashmere fibre growth and moult. So, thePRL gene is a potential candidate gene for cashmere traits in marker-assisted selection (MAS). In this paper, we report a novel missense single-nucleotide polymorphism (SNP) within the goatPRL gene in 1367 individuals by PCR-SSCP (polymerase chain reaction with single-strand conformation polymorphism) analysis and DNA sequencing. The novel X76049:g.576C>A mutation is confirmed byEco24I PCR-RFLP (restriction fragment length polymorphism) analysis and causes a missense codon (Pro176Thr). The frequencies of allele C varied from 0.79 to 0.93 in 9 analysed goat populations. C allele was correlated with higher fibre length (P = 0.014).
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References
Aravindakshan TV, 2005. Polymorphism at K-casein and prolactin loci in goats investigated by PCR-RFLP and DNA sequencing. Indian Vet J 82: 126–130.
Dicks P, 1994. The role of prolactin and melatonin in regulating the timing of the spring moult in the cashmere goat. European Fine Fire Network, Occasional Publication 2: 109–127.
Gan QF, Zhang LP, Li JY, Hou GY, Li HD, Gao X, et al. 2008. Association analysis of thyroglobulin gene variants with carcass and meat quality traits in beef cattle. J Appl Genet 49: 251–255.
Lan XY, Pan CY, Chen H, Zhan CL, Li JY, Zhao M, et al. 2007a. An AluI PCR-RFLP detecting a silent allele at the goat POU1F1 locus and its association with production traits. Small Ruminant Research 73: 8–12.
Lan XY, Pan CY, Chen H, Lei CZ, Hua LS, Yang XB, et al. 2007b. DdeI Polymorphism in coding region of goat POU1F1 gene and its association with production traits. Asian-Aust. J Anim Sci 20: 1342–1348.
Li JT, Wang AH, Chen P, Li HB, Zhang CS, Du LX, 2006. Relationship between the polymorphisms of 5’ regulation region of prolactin gene and milk traits in Chinese Holstein dairy cows. Asian-Aust J Anim Sci 19: 459–462.
Lincoln GA, 1990. Correlation with changes in horns and pelage, but not reproduction, of seasonal cycles in the secretion of prolactin in rams of wild, feral and domesticated breeds of sheep. J Reprod Fertil 90: 285–296.
Müllenbach R, Lagoda PJ, Welter C, 1989. An efficient salt-chloroform extraction of DNA from blood and tissue. Trends Genet 5: 391.
Rhind SM, Kyle CE, Duff EI, 2004. Effects of season and of manipulation of circulating prolactin concentrations on deiodinase activity in cashmere goat skin. Aust J Agr Res 55: 211–221.
Rose J, Kennedy M, Johnston B, Foster W, 1998. Serum prolactin and dehydroepiandrosterone concentrations during the summer and winter hair growth cycles of mink (Mustela vison). Comp Biochem Phys A 121: 263–371.
Santiago-Moreno J, López-Sebastián A, del Campo A, González-Bulnes A, Picazo, Gómez-Brunet A, 2004. Effect of constant-release melatonin implants and prolonged exposure to a long day photoperiod on prolactin secretion and hair growth in mouflon (Ovis gmelini musimon). Domest Anim Endocrin 26: 303–314.
Vincent AL, Rothschild MF, 1997. A restriction fragment length polymorphism in the ovine prolactin gene. J Anim Sci 75: 1686–1686.
Zhou HM, Allain D, Li JQ, Zhang WG, Yu XC, 2003. Effects of non-genetic factors on production traits of Inner Mongolia cashmere goats in China. Small Rumin Res 47: 85–89.
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Lan, X.Y., Pan, C.Y., Chen, H. et al. Novel SNP of the goatprolactin gene (PRL) associated with cashmere traits. J Appl Genet 50, 51–54 (2009). https://doi.org/10.1007/BF03195652
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DOI: https://doi.org/10.1007/BF03195652