Human adult mesenchymal stem or stromal cells (h-MSC) therapy has gained considerable attention due to the potential to treat or cure diseases given their immunosuppressive properties and tissue regeneration capabilities. Researchers have explored diverse strategies to promote high h-MSC production without losing functional characteristics or properties. Physical stimulus including stiffness, geometry, and topography, chemical stimulus, like varying the surface chemistry, and biochemical stimuli such as cytokines, hormones, small molecules, and herbal extracts have been studied but have yet to be translated to industrial manufacturing practice. In this review, we describe the role of those stimuli on h-MSC manufacturing, and how these stimuli positively promote h-MSC properties, impacting the cell manufacturing field for cell-based therapies. In addition, we discuss other process considerations such as bioreactor design, good manufacturing practice, and the importance of the cell donor and ethics factors for manufacturing potent h-MSC.

中文翻译：

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物理、化学和生物刺激对 h-MSC 扩增及其功能特征的影响。


人类间充质干细胞或基质细胞（h-MSC）疗法因其免疫抑制特性和组织再生能力而具有治疗或治愈疾病的潜力，因此受到了广泛关注。研究人员探索了多种策略来促进 h-MSC 的高产量，同时又不损失功能特征或特性。物理刺激（包括刚度、几何形状和形貌）、化学刺激（如改变表面化学）和生化刺激（如细胞因子、激素、小分子和草药提取物）已被研究，但尚未转化为工业制造实践。在这篇综述中，我们描述了这些刺激对 h-MSC 制造的作用，以及这些刺激如何积极促进 h-MSC 特性，影响基于细胞的疗法的细胞制造领域。此外，我们还讨论了其他工艺注意事项，例如生物反应器设计、良好生产规范、细胞供体的重要性以及制造有效 h-MSC 的伦理因素。