Two-component systems (TCSs) are highly conserved in prokaryotes, endowing cells with multiple physiological functions to respond to changes in the ambient environment. The signaling pathway of a typical TCS consists of a sensory histidine kinase and a response regulator. The TCSs of Kocuria rhizophila, which is usually used as a target strain for various antibiotics and other adverse factors, have captured our interest due to their potential roles in bacterial adaptation for survival. Herein, the distribution and putative biological functions of the TCSs of K. rhizophila DC2201 were analyzed by using bioinformatics, and a preliminary TCS regulatory network was constructed. A representative and important TCS (i.e., HK8700–RR8701 system), which is homologous to the LiaS–LiaR system previously discovered in Bacillus subtilis, was identified and characterized through yeast two-hybrid screening and phosphorylation assays. Detailed information of TCSs is expected to offer novel insights into the adaptation mechanism of K. rhizophila and thus boost its application.

中文翻译：

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鉴定和鉴定科氏菌DC2201的两组分系统HK8700-RR8701。

两组分系统（TCS）在原核生物中高度保守，赋予细胞多种生理功能，以响应周围环境的变化。典型TCS的信号传导途径由感觉组氨酸激酶和响应调节剂组成。经常用作各种抗生素和其他不利因素的靶菌株的库斯科菌属的TCS，由于它们在细菌适应生存中的潜在作用而引起了我们的兴趣。在此，嗜酸K.rhiophila的TCS的分布和推测的生物学功能利用生物信息学技术对DC2201进行了分析，并建立了初步的TCS调节网络。通过酵母双杂交筛选和磷酸化分析鉴定并鉴定了具有代表性的重要TCS（即HK8700-RR8701系统），该系统与以前在枯草芽孢杆菌中发现的LiaS-LiaR系统同源。预期TCS的详细信息将为对K. rhizophila的适应机制提供新颖的见解，从而促进其应用。