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Complete genomic sequence of tea-oil camellia deltapartitivirus 1, a novel virus from Camellia oleifera.
Archives of Virology ( IF 2.7 ) Pub Date : 2019-10-28 , DOI: 10.1007/s00705-019-04429-0
L-P Wu 1 , Y-M Du 1 , H Xiao 1 , L Peng 1 , R Li 2
Affiliation  

Three viral contig sequences, which represented complete genome of a novel virus with three dsRNAs of 1,712 nucleotides (nt) (dsRNA1), 1,504 nt (dsRNA2) and 1,353 nt (dsRNA3), were found in tea-oil camellia plants by high-throughput sequencing analysis. The three dsRNAs were re-sequenced by RT-PCR cloning. The largest dsRNA, dsRNA1, had a single open reading frame (ORF) that encoded a putative 52.7-kDa protein of a putative viral RNA-dependent RNA polymerase (RdRp). DsRNA2 and dsRNA3 were predicted to encode putative capsid proteins (CPs) of 40.47 kDa and 40.59 kDa, respectively. The virus, which is provisionally named "tea-oil camellia deltapartitivirus 1", shared amino acid sequence itentities of 36.09-69.18% with members of the genus Deltapartitivirus on RdRp. Phylogenetic analysis based on RdRp also placed the new virus and other deltapartitiviruses together in a group, suggesting that this virus should be considered a new member of the genus Deltapartitivirus.

中文翻译:

茶油山茶deltapartitivirus 1(一种来自山茶的新型病毒)的完整基因组序列。

通过高通量在茶油茶树植物中发现了三个病毒重叠群序列,它们代表一种新型病毒的完整基因组,具有三个dsRNA,分别为1,712个核苷酸(nt)(dsRNA1),1,504 nt(dsRNA2)和1,353 nt(dsRNA3)。测序分析。通过RT-PCR克隆对这三个dsRNA进行了重新测序。最大的dsRNA dsRNA1具有一个开放阅读框(ORF),其编码假定的病毒RNA依赖性RNA聚合酶(RdRp)的假定的52.7 kDa蛋白。预测DsRNA2和dsRNA3分别编码40.47 kDa和40.59 kDa的假定衣壳蛋白(CPs)。该病毒临时命名为“茶油山茶三角洲分枝病毒1”,与RdRp上的三角洲分枝病毒属成员共享36.09-69.18%的氨基酸序列。
更新日期:2020-01-13
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