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Complete genomic sequence of tea-oil camellia deltapartitivirus 1, a novel virus from Camellia oleifera

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Abstract

Three viral contig sequences, which represented complete genome of a novel virus with three dsRNAs of 1,712 nucleotides (nt) (dsRNA1), 1,504 nt (dsRNA2) and 1,353 nt (dsRNA3), were found in tea-oil camellia plants by high-throughput sequencing analysis. The three dsRNAs were re-sequenced by RT-PCR cloning. The largest dsRNA, dsRNA1, had a single open reading frame (ORF) that encoded a putative 52.7-kDa protein of a putative viral RNA-dependent RNA polymerase (RdRp). DsRNA2 and dsRNA3 were predicted to encode putative capsid proteins (CPs) of 40.47 kDa and 40.59 kDa, respectively. The virus, which is provisionally named “tea-oil camellia deltapartitivirus 1”,  shared amino acid sequence itentities of 36.09-69.18% with members of the genus Deltapartitivirus on RdRp. Phylogenetic analysis based on RdRp also placed the new virus and other deltapartitiviruses together in a group, suggesting that this virus should be considered a new member of the genus Deltapartitivirus.

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Acknowledgements

This work was supported by the U.S. Department of Agriculture, Agriculture Research Service, under research project 1215-22000-302-00D “Characterizing and Detecting Pathogens to Ensure Safe Exchange of Plant Germplasm” and the National Natural Science Foundation of China (Grant no. 31860525).

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  1. L.-P. Wu and Y.-M. Du are co-first authors.

Authors and Affiliations

  1. School of Life Science, Key Laboratory of Poyang Lake Environment and Resource, Ministry of Education, Nanchang University, Nanchang, Jiangxi, 330031, China

    L.-P. Wu, Y.-M. Du, H. Xiao & L. Peng

  2. USDA-ARS, National Germplasm Resources Laboratory, Beltsville, MD, 20705, USA

    R. Li

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  1. L.-P. Wu
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Correspondence to R. Li.

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Wu, LP., Du, YM., Xiao, H. et al. Complete genomic sequence of tea-oil camellia deltapartitivirus 1, a novel virus from Camellia oleifera. Arch Virol 165, 227–231 (2020). https://doi.org/10.1007/s00705-019-04429-0

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  • DOI: https://doi.org/10.1007/s00705-019-04429-0

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