Mungbean (Vigna radiata) and ricebean (V. umbellata) were utilized to obtain an inter-specific recombinant inbred line (RIL) population with the objective of detecting quantitative trait loci (QTL) associated with mungbean yellow mosaic virus (MYMV) resistance. To precisely map QTLs, accurate genetic linkage maps are essential. In the present study, genotyping-by-sequencing (GBS) platform was utilized to develop the genetic linkage map. The map contained 538 single nucleotide polymorphism (SNP) markers, consisted of 11 linkage groups and spanned for 1291.7 cM with an average marker distance of 2.40 cM. The individual linkage group ranged from 90.2 to 149.1 cM in length, and the SNP markers were evenly distributed in the genetic linkage map, with 30–79 SNP markers per chromosome. The QTL analysis using the genetic map and 2 years (2015 and 2016) of phenotyping data identified five QTLs with phenotypic variation explained (PVE) from 10.11 to 20.04%. Of these, a QTL on chromosome 4, designated as qMYMV4-1, was major and stably detected in the same marker interval in both years. This QTL region harbours possible candidate genes for controlling MYMV resistance. The linkage map and QTL/gene (s) for MYMV resistance identified in this study should be useful for QTL fine mapping and cloning for further studies.

中文翻译：

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使用Vigna radiata×Vigna umbellata的种间杂交检测与绿豆黄花叶病毒（MYMV）抗性相关的QTL。

绿豆（Vigna radiata）和bean豆（V. umbellata）为了检测与绿豆黄花叶病毒（MYMV）抗性相关的定量性状基因座（QTL），利用cDNA来获得种间重组近交系（RIL）种群。要精确定位QTL，准确的遗传连锁图至关重要。在本研究中，通过测序进行基因分型（GBS）平台来开发遗传连锁图谱。该图包含538个单核苷酸多态性（SNP）标记，由11个连锁基团组成，跨度为1291.7 cM，平均标记距离为2.40 cM。单个连锁组的长度范围为90.2至149.1 cM，SNP标记在遗传连锁图中平均分布，每个染色体30-79个SNP标记。使用遗传图谱和2年（2015年和2016年）的表型数据进行的QTL分析确定了5个表型变异解释（PVE）从10.11％到20.04％的QTL。其中，第4号染色体上的QTL指定为两年中，qMYMV4-1主要且稳定，在相同的标记间隔内被检测到。该QTL区包含可能的候选基因，用于控制MYMV抗性。在这项研究中鉴定的MYMV抗性的连锁图谱和QTL /基因应该对QTL精细定位和克隆进一步研究有用。