The regulation by adenylates of activities of various partial electron transport systems in spinach chloroplasts was studied using systems from H2O to 2,5-dimethyl-p-benzoquinone, H2O to 2,6-dichlorophenolindophenol, reduced 2,6-dichlorophenolindophenol to methyl viologen, and H2O to methyl viologen or ferricyanide. Adenylates regulated all of them. The ratio of the amount of esterified Pi (P) to that of electrons transported (Deltae) in coupling with phosphorylation manifested that there are two phosphorylation sites: one between H2O and 2,5-dimethyl-p-benzoquinone or 2,6-dichlorophenolindophenol and another between reduced 2,6-dichlorophenolindophenol and methyl viologen, under the proposed stoichiometries, i.e., P/DeltaH+ = 0.5 and DeltaH+/Deltae = 1, where DeltaH+ is the amount of protons pumped by electron transport (= those translocated during phosphorylation), when the basal electron transport (the part not regulated by adenylates) was excluded. The effects of pH, phlorizin, and methylamine on the adenylate regulation of electron transport, and the stimulation profile of electron transport coupled with quasiarsenylation suggested no distinction between the two phosphorylation sites.

中文翻译：

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光合电子传递的腺苷酸调节和菠菜叶绿体磷酸化的耦合位点。

使用从 H2O 到 2,5-二甲基-对苯醌、H2O 到 2,6-二氯苯酚吲哚酚、将 2,6-二氯苯酚吲哚酚还原为甲基紫精、和 H2O 转化为甲基紫精或铁氰化物。腺苷酸调节所有这些。与磷酸化耦合的酯化 Pi 量 (P) 与电子传输量 (Deltae) 之比表明存在两个磷酸化位点：一个位于 H2O 和 2,5-二甲基-对苯醌或 2,6-二氯苯酚吲哚酚之间另一个介于还原的 2,6-二氯苯酚和甲基紫精之间，在建议的化学计量下，即 P/DeltaH+ = 0.5 和 DeltaH+/Deltae = 1，其中 DeltaH+ 是在排除基础电子传输（不受腺苷酸调节的部分）时由电子传输泵送的质子量（= 磷酸化过程中易位的质子）。pH、根皮苷和甲胺对电子传递的腺苷酸调节的影响，以及电子传递与准砷化的刺激谱表明两个磷酸化位点之间没有区别。