Ligand binding RNAs such as artificially created RNA-aptamers are structurally highly diverse. Therefore, they represent important model systems for investigating RNA-folding, RNA-dynamics and the molecular recognition of chemically very different ligands, ranging from small molecules to whole cells. High-resolution structures of RNA-aptamers in complex with their cognate ligands often reveal unexpected tertiary structure elements. Recent studies on different classes of aptamers binding the nucleotide triphosphate GTP as a ligand showed that these systems not only differ widely in binding affinity but also in their ligand binding modes and structural complexity. We initiated the NMR-based structure determination of the high-affinity binding GTP-aptamer 9-12 in order to gain further insights into the diversity of ligand binding modes and structural variability of those aptamers. Here, we report ¹H, ¹³C and ¹⁵N resonance assignments for the GTP 9-12-aptamer bound to GTP as the prerequisite for the structure determination by solution NMR.

中文翻译：

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与GTP结合的GTP结合RNA适体9-12的NMR共振分配。

配体结合RNA（例如人工创建的RNA适体）在结构上高度多样。因此，它们代表了重要的模型系统，用于研究RNA折叠，RNA动力学以及化学上非常不同的配体（从小分子到整个细胞）的分子识别。RNA适体的高分辨率结构及其同源配体通常显示出出乎意料的三级结构元素。对结合核苷酸三磷酸GTP作为配体的不同种类的适体的最新研究表明，这些系统不仅在结合亲和力方面而且在它们的配体结合方式和结构复杂性方面差异很大。我们启动了高亲和力结合GTP适体9-12的基于NMR的结构确定，以便进一步了解配体结合模式的多样性和这些适体的结构变异性。在这里，我们报告与GTP结合的GTP 9-12-适体的¹ H，¹³ C和¹⁵ N共振分配是通过溶液NMR确定结构的前提条件。