Zinc deficiency in aquatic animals affects the biological processes and physiological functions. Thus, the supplement of ZnONPs can be used as an alternative method to overcome zinc deficiency. Nanoparticles have the potential to enhance the growth and health of the fish. The main aim of this study is to evaluate the growth efficacy of ZnONP-supplemented diet with fingerlings of Labeo rohita. The green synthesized ZnONPs were characterized by ultraviolet-visible (UV-Vis) spectroscopy, Fourier transformer infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS). Different concentrations of ZnONPs (5, 7.5, and 10 mg/kg) were administered in the basal diet of freshwater fish Labeo rohita for 45 days to observe the growth and metabolic functions of the body. However, the fish fed with 10 mg/kg ZnONP-supplemented diet shows that the growth performance was highly increased followed by 7.5 mg/kg and 5 mg/kg ZnONPs when compared with the control. The biochemical, hematological, and digestive enzyme activities were also significantly increased with different concentrations of ZnONPs. The effects of zinc oxide nanoparticles show the higher improvement of growth and metabolic functions in Labeo rohita. These results suggest that the nanotechnology could apply for feed formulation technology and pave the way for the dietary supplementation of zinc oxide nanoparticles as safe ingredients for aquatic animals to overcome the zinc deficiency.

中文翻译：

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绿色合成的氧化锌纳米颗粒作为饲料添加剂，可改善淡水鱼Labeo rohita的生长，生化和血液学参数。

水生动物缺锌会影响其生物学过程和生理功能。因此，ZnONP的补充可以用作克服锌缺乏的替代方法。纳米颗粒具有增强鱼类生长和健康的潜力。这项研究的主要目的是评估ZnONP补充日粮与Labeo rohita的鱼苗的生长功效。绿色合成的ZnONPs的特征在于紫外可见光谱（UV-Vis），傅立叶变换红外光谱（FTIR），X射线衍射（XRD），扫描电子显微镜（SEM）和能量色散光谱（EDS）。在淡水鱼Labeo rohita的基础饮食中施用不同浓度的ZnONPs（5、7.5和10 mg / kg）45天，以观察人体的生长和代谢功能。然而，饲喂补充了10 mg / kg ZnONP的饲料的鱼显示，与对照组相比，其生长性能大大提高，其后为7.5 mg / kg和5 mg / kg ZnONP。不同浓度的ZnONPs的生化，血液和消化酶活性也显着提高。氧化锌纳米颗粒的作用表明在Labeo rohita中具有更高的生长和代谢功能。这些结果表明，纳米技术可以应用于饲料配方技术，并为膳食补充氧化锌纳米颗粒作为水生动物克服锌缺乏的安全成分铺平了道路。不同浓度的ZnONPs的生化，血液和消化酶活性也显着提高。氧化锌纳米颗粒的作用表明在Labeo rohita中具有更高的生长和代谢功能。这些结果表明，纳米技术可以应用于饲料配方技术，并为膳食补充氧化锌纳米颗粒作为水生动物克服锌缺乏的安全成分铺平了道路。不同浓度的ZnONPs的生化，血液和消化酶活性也显着提高。氧化锌纳米颗粒的作用表明在Labeo rohita中具有更高的生长和代谢功能。这些结果表明，纳米技术可以应用于饲料配方技术，并为膳食补充氧化锌纳米颗粒作为水生动物克服锌缺乏的安全成分铺平了道路。