The objective of the present study was to assess hair essential and toxic trace elements and minerals in children with cerebral palsy in relation to age of the examinees. A total of 70 children with cerebral palsy and 70 healthy controls aged 0-4 years old were enrolled in the present study. The examined children were also divided into two age groups of those younger and older than 2 years old. Hair trace element content was assessed using ICP-MS at NexION 300D (PerkinElmer, USA). The obtained data demonstrate that hair boron was more than 2-fold lower in CP children as compared with the control group. At the same time, hair Na, Se, and V levels were 21%, 12%, and 20% lower when compared with healthy controls, respectively. It is also notable that a 9% and 28% decrease in hair Fe and Li levels respectively were nearly significant. The observed alterations were more profound in a younger group of patients. No significant group difference in hair toxic metal and metalloid levels was observed between the general cohorts of children with and without CP. In regression models, only hair Al and Ca contents were significantly associated with the presence of cerebral palsy, whereas hair Mg, Na, Ni, and Se levels were characterized as significant negative predictors. The observed alteration in trace element metabolism may also provide an additional link between cerebral palsy, psychomotor delay, and certain diseases, including diabetes, epilepsy, and osteoporosis. However, further studies using other substrates (blood, urine) or biomarkers are required.

中文翻译：

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0-4岁脑瘫儿童头发中必需和有毒微量元素和矿物质的病例对照研究。

本研究的目的是评估脑瘫患儿与受检者年龄有关的头发必需和有毒微量元素及矿物质。本研究共纳入70名脑瘫患儿和70名0-4岁的健康对照。被检查的孩子也分为两个年龄段，分别是年龄小于2岁的孩子。使用NexION 300D（美国PerkinElmer）的ICP-MS评估头发中的微量元素含量。获得的数据表明，与对照组相比，CP儿童的发硼降低了2倍以上。同时，与健康对照组相比，头发的Na，Se和V水平分别降低了21％，12％和20％。还值得注意的是，头发中的铁和锂含量分别下降了9％和28％，几乎是显着的。在年轻的患者组中观察到的变化更为深刻。在有和没有CP的儿童的一般队列之间，在头发有毒金属和准金属水平上没有观察到显着的群体差异。在回归模型中，只有头发中的Al和Ca含量与脑瘫显着相关，而头发中Mg，Na，Ni和Se的含量则是显着的阴性预测指标。观察到的微量元素代谢的改变也可能在脑瘫，精神运动延迟和某些疾病（包括糖尿病，癫痫病和骨质疏松症）之间提供额外的联系。但是，需要使用其他底物（血液，尿液）或生物标记物进行进一步研究。在有和没有CP的儿童的一般队列之间，在头发有毒金属和准金属水平上没有观察到显着的群体差异。在回归模型中，只有头发中的Al和Ca含量与脑瘫显着相关，而头发中Mg，Na，Ni和Se的含量则是显着的阴性预测指标。观察到的微量元素代谢的改变也可能在脑瘫，精神运动延迟和某些疾病（包括糖尿病，癫痫病和骨质疏松症）之间提供额外的联系。但是，需要使用其他底物（血液，尿液）或生物标记物进行进一步研究。在有和没有CP的儿童的一般队列之间，在头发有毒金属和准金属水平上没有观察到显着的群体差异。在回归模型中，只有头发中的Al和Ca含量与脑瘫显着相关，而头发中的Mg，Na，Ni和Se水平则被认为是显着的阴性指标。观察到的微量元素代谢的改变也可能在脑瘫，精神运动延迟和某些疾病（包括糖尿病，癫痫病和骨质疏松症）之间提供额外的联系。但是，需要使用其他底物（血液，尿液）或生物标记物进行进一步研究。Na，Ni和Se水平被确定为显着的阴性预测指标。观察到的微量元素代谢的改变也可能在脑瘫，精神运动延迟和某些疾病（包括糖尿病，癫痫病和骨质疏松症）之间提供额外的联系。但是，需要使用其他底物（血液，尿液）或生物标记物进行进一步研究。Na，Ni和Se水平被确定为显着的阴性预测指标。观察到的微量元素代谢的改变也可能在脑瘫，精神运动延迟和某些疾病（包括糖尿病，癫痫病和骨质疏松症）之间提供额外的联系。但是，需要使用其他底物（血液，尿液）或生物标记物进行进一步研究。