Ovarian cancer is considered to be the most lethal type of gynecological cancer. During the advanced stages of ovarian cancer, an accumulation of ascites is observed. Fucosylation has been classified as an abnormal post-translational modification that is present in many diseases, including ovarian cancer. Ovarian cancer cells that are cultured with ascites stimulation change their morphology; concomitantly, the fucosylation process is altered. However, it is not known which fucosylated proteins are modified. The goal of this work was to identify the differentially fucosylated proteins that are expressed by ovarian cancer cell lines that are cultured with ovarian cancer patients’ ascites. Aleuria aurantia lectin was used to detect fucosylation, and some changes were observed, especially in the cell membrane. Affinity chromatography and mass spectrometry (MALDI-TOF) were used to identify 6 fucosylated proteins. Four proteins (Intermediate filament family orphan 1 [IFFO1], PHD finger protein 20-like protein 1 [PHF20L1], immunoglobulin gamma 1 heavy chain variable region partial [IGHV1–2], and Zinc finger protein 224 [ZNF224]) were obtained from cell cultures stimulated with ascites, and the other two proteins (Peregrin [BRPF1] and Dystrobrevin alpha [DTNA]) were obtained under normal culture conditions. The fucosylated state of some of these proteins was further analyzed. The experimental results show that the ascites of ovarian cancer patients modulated the fucosylation process. The PHD finger protein 20-like protein 1, Zinc finger protein 224 and Peregrin proteins colocalize with fucosylation at different levels.

卵巢癌被认为是最致命的妇科癌症。在卵巢癌晚期，会观察到腹水积聚。岩藻糖基化被归类为一种异常翻译后修饰，存在于许多疾病中，包括卵巢癌。用腹水刺激培养的卵巢癌细胞会改变其形态；与此同时，岩藻糖基化过程也发生改变。然而，尚不清楚哪些岩藻糖基化蛋白质被修饰。这项工作的目标是鉴定用卵巢癌患者腹水培养的卵巢癌细胞系表达的差异岩藻糖基化蛋白。使用Aleuria aurantia凝集素检测岩藻糖基化，观察到一些变化，特别是在细胞膜上。使用亲和色谱和质谱 (MALDI-TOF) 鉴定 6 种岩藻糖基化蛋白。四种蛋白（中间丝家族孤儿 1 [IFFO1]、PHD 指蛋白 20 样蛋白 1 [PHF20L1]、免疫球蛋白 γ 1 重链可变区部分 [IGHV1–2] 和锌指蛋白 224 [ZNF224]）获自用腹水刺激细胞培养物，其他两种蛋白（Peregrin [BRPF1] 和 Dystrobrevin alpha [DTNA]）是在正常培养条件下获得的。进一步分析了其中一些蛋白质的岩藻糖基化状态。实验结果表明，卵巢癌患者的腹水调节岩藻糖基化过程。 PHD 指蛋白 20 样蛋白 1、锌指蛋白 224 和 Peregrin 蛋白在不同水平上与岩藻糖基化共定位。