The effects of fructose on plantaricin Q7 biosynthesis in Lactobacillus plantarum (L. plantarum) Q7 were investigated. Fructose induced and accelerated plantaricin Q7 production in the early stages of biosynthesis. Two-dimensional electrophoresis and quantitative real-time reverse transcriptase PCR (qRT-PCR) revealed that shock-related proteins were overexpressed in L. plantarum grown in MRS-fructose compared with cultures grown in MRS broth. A preheat-shock (PHS) treatment was used to overexpress shock-related proteins to enhance plantaricin Q7 production. The final plantaricin Q7 level reached 2873.08 IU/mL following PHS treatment, which was 23.36% higher than that obtained using a control strategy without PHS treatment. qRT-PCR analyses indicated that only groL was induced (by 10-fold) following PHS treatment. Evidently, groL plays a critical role in plantaricin Q7 biosynthesis. These results demonstrate that PHS treatment is an efficient approach to improving plantaricin Q7 production. The information obtained in this study may increase the amount of efficient antimicrobial peptide production using L. plantarum.

中文翻译：

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果糖和休克相关基因groL的过表达对Plant藤素Q7产量的影响。

果糖对plantaricin Q7生物合成的影响植物乳杆菌（植物乳杆菌）Q7进行了调查。在生物合成的早期阶段，果糖诱导并加速了plant那藤Q7的生产。二维电泳和实时定量逆转录PCR（qRT-PCR）表明，休克相关蛋白在植物乳杆菌中过表达与在MRS肉汤中培养的培养物相比，在MRS-果糖中生长的培养物。预热休克（PHS）处理用于过度表达休克相关蛋白，以增强plant藤素Q7的产生。PHS处理后的最终plant草素Q7水平达到2873.08 IU / mL，比不使用PHS处理的对照策略高23.36％。qRT-PCR分析表明，PHS处理后仅诱导了groL（10倍）。显然，groL在plant草素Q7生物合成中起关键作用。这些结果表明，PHS处理是提高plant那霉素Q7产量的有效方法。在这项研究中获得的信息可能会增加使用植物乳杆菌的有效抗菌肽的生产量。