Ophiocordyceps sinensis is a natural fungus that has been valued as a health food and used in traditional Chinese medicine for centuries. The fungus is parasitic and colonizes insect larva. Naturally occurring O. sinensis thrives at high altitude in cold and grassy alpine meadows on the Himalayan mountain ranges. Wild Ophiocordyceps is becoming increasingly rare in its natural habitat, and its price limits its use in clinical practice. Therefore, the development of a standardized alternative is a great focus of research to allow the use of Ophiocordyceps as a medicine. To develop an alternative for wild Ophiocordyceps, a refined standardized extract, CBG-CS-2, was produced by artificial fermentation and extraction of the mycelial strain Paecilomyces hepiali CBG-CS-1, which originated from wild O. sinensis. In this study, we analyzed the in vitro immune-modulating effect of CBG-CS-2 on natural killer cells and B and T lymphocytes. CBG-CS-2 stimulated splenocyte proliferation and enhanced Th1-type cytokine expression in the mouse splenocytes. Importantly, in vitro CBG-CS-2 treatment enhanced the killing activity of the NK-92MI natural killer cell line. These results indicate that the mycelial culture extract prepared from Ophiocordyceps exhibits immune-modulating activity, as was observed in vivo and this suggests its possible use in the treatment of diseases caused by abnormal immune function.

中文翻译：

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使用从冬虫夏草菌丝培养物中制备的提取物对 NK 细胞和淋巴细胞的体外刺激

冬虫夏草是一种天然真菌，几个世纪以来一直被视为保健食品和用于中药。这种真菌是寄生的，并定植于昆虫幼虫。天然存在的 O. sinensis 在喜马拉雅山脉寒冷多草的高山草甸的高海拔地区茁壮成长。野生虫草在其自然栖息地变得越来越稀有，其价格限制了其在临床实践中的使用。因此，标准化替代品的开发是研究的一个重点，以允许使用虫草作为药物。为了开发野生冬虫夏草的替代品，一种精制的标准化提取物 CBG-CS-2 是通过人工发酵和提取源自野生冬虫夏草的菌丝株 Paecilomyces hepiali CBG-CS-1 生产的。在这项研究中，我们分析了 CBG-CS-2 对自然杀伤细胞以及 B 和 T 淋巴细胞的体外免疫调节作用。CBG-CS-2 刺激脾细胞增殖并增强小鼠脾细胞中 Th1 型细胞因子的表达。重要的是，体外 CBG-CS-2 处理增强了 NK-92MI 自然杀伤细胞系的杀伤活性。这些结果表明，从虫草属制备的菌丝体培养提取物表现出免疫调节活性，正如在体内观察到的那样，这表明它可能用于治疗由异常免疫功能引起的疾病。体外 CBG-CS-2 处理增强了 NK-92MI 自然杀伤细胞系的杀伤活性。这些结果表明，从虫草属制备的菌丝体培养提取物表现出免疫调节活性，正如在体内观察到的那样，这表明它可能用于治疗由异常免疫功能引起的疾病。体外 CBG-CS-2 处理增强了 NK-92MI 自然杀伤细胞系的杀伤活性。这些结果表明，从虫草属制备的菌丝培养提取物表现出免疫调节活性，正如在体内观察到的那样，这表明它可能用于治疗由异常免疫功能引起的疾病。