Soybean rust caused by Phakopsora pachyrhizi Sydow is a devastating foliar disease that has spread to most soybean growing regions throughout the world, including the USA. Four independent rust resistance genes, Rpp1-Rpp4, have been identified in soybean that recognize specific isolates of P. pachyrhizi. A suppressive subtraction hybridization (SSH) complementary DNA (cDNA) library was constructed from the soybean accession PI200492, which contains Rpp1, after inoculation with two different isolates of P. pachyrhizi that result in susceptible or immune reactions. Both forward and reverse SSH were performed using cDNA from messenger RNA pooled from 1, 6, 12, 24, and 48 h post-inoculation. A total of 1,728 SSH clones were sequenced and compared to sequences in GenBank for similarity. Microarray analyses were conducted on a custom 7883 soybean-cDNA clone array encompassing all of the soybean-rust SSH clones and expressed sequence tags from four other soybean cDNA libraries. Results of the microarray revealed 558 cDNA clones differentially expressed in the immune reaction. The majority of the upregulated cDNA clones fell into the functional category of defense. In particular, cDNA clones with similarity to peroxidases and lipoxygenases were prevalent. Downregulated cDNA clones included those with similarity to cell-wall-associated protein, such as extensins, proline-rich proteins, and xyloglucan endotransglycosylases.

中文翻译：

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大豆中对豆薯层锈菌的抗性表达模式揭示了过氧化物酶和脂氧合酶的重要性。


由豆薯层锈菌引起的大豆锈病是一种毁灭性的叶部病害，已蔓延到包括美国在内的世界上大多数大豆种植区。已在大豆中鉴定出四个独立的锈病抗性基因 Rpp1-Rpp4，它们可识别豆薯的特定分离株。在接种两种不同的豆薯赤霉分离株后，从大豆登记号 PI200492 构建抑制性消减杂交 (SSH) 互补 DNA (cDNA) 文库，其中包含 Rpp1，导致敏感或免疫反应。正向和反向 SSH 均使用接种后 1、6、12、24 和 48 小时汇集的信使 RNA 的 cDNA 进行。对总共 1,728 个 SSH 克隆进行了测序，并与 GenBank 中的序列进行了相似性比较。在定制的 7883 大豆 cDNA 克隆阵列上进行微阵列分析，该阵列包含所有大豆锈菌 SSH 克隆和来自四个其他大豆 cDNA 文库的表达序列标签。微阵列结果显示，558 个 cDNA 克隆在免疫反应中差异表达。大多数上调的 cDNA 克隆属于防御功能类别。特别是，与过氧化物酶和脂氧合酶相似的cDNA克隆很普遍。下调的 cDNA 克隆包括与细胞壁相关蛋白相似的克隆，例如延伸蛋白、富含脯氨酸的蛋白和木葡聚糖内转糖基酶。