The complete coding sequences of porcine genesNCF2, BCKDHB andBCKDHA were amplified by using reverse transcriptase polymerase chain reaction (RT-PCR), basing on the conserved coding sequence information of humans or other mammals. These 3 novel porcine genes were then assigned GeneIDs: 100142665, 100142669 and 100142666. The phylogenetic tree analysis revealed that the porcineNCF2, BCKDHB andBCKDHA all are most closely related to the bovineNCF2, BCKDHB andBCKDHA. Tissue expression profile analysis revealed that porcineNCF2, BCKDHB andBCKDHA genes were differentially expressed in tissues, including skeletal muscle, the heart, liver, fat, kidney, lung, small and large intestine.

中文翻译：

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3 种新型猪基因的分离、序列鉴定和组织表达谱：NCF2、BCKDHB 和 BCKDHA。

根据人类或其他哺乳动物的保守编码序列信息，采用逆转录聚合酶链反应（RT-PCR）方法扩增了猪基因NCF2、BCKDHB和BCKDHA的完整编码序列。然后将这 3 个新的猪基因分配为 GeneID：100142665、100142669 和 100142666。系统发育树分析显示，猪NCF2、BCKDHB和BCKDHA都与牛NCF2、BCKDHB和BCKDHA 的亲缘关系最密切。组织表达谱分析表明，猪NCF2、BCKDHB和BCKDHA 基因在组织中差异表达，包括骨骼肌、心脏、肝脏、脂肪、肾脏、肺、小肠和大肠。