Abstract
The complete coding sequences of porcine genesNCF2, BCKDHB andBCKDHA were amplified by using reverse transcriptase polymerase chain reaction (RT-PCR), basing on the conserved coding sequence information of humans or other mammals. These 3 novel porcine genes were then assigned GeneIDs: 100142665, 100142669 and 100142666. The phylogenetic tree analysis revealed that the porcineNCF2, BCKDHB andBCKDHA all are most closely related to the bovineNCF2, BCKDHB andBCKDHA. Tissue expression profile analysis revealed that porcineNCF2, BCKDHB andBCKDHA genes were differentially expressed in tissues, including skeletal muscle, the heart, liver, fat, kidney, lung, small and large intestine.
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Liu, G.Y. Isolation, sequence identification, and tissue expression profile of 3 novel porcine genes:NCF2, BCKDHB andBCKDHA . J Appl Genet 50, 47–50 (2009). https://doi.org/10.1007/BF03195651
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DOI: https://doi.org/10.1007/BF03195651