The paper presents data on the cultivation of human dermal fibroblasts and rabbit mesenchymal stromal cells on two types of porous titanium implants, i.e., those with irregular pores formed by pressed titanium particles and those with regular pores formed by the cohesion of one-size titanium particles inside the implant. The goal of this study was to determine what type of titanium implant porosity ensured its strongest interaction with cells. Cells were cultivated on implants for 7 days. During this period, they formed a confluent monolayer on the implant surface. Cells grown on titanium implants were monitored by scanning electron microscopy. Fibroblasts interaction with implants depended on the implant porosity structure. On implants with irregular pores cells were more spread. On implants with regular pores fibroblasts enveloped particles and were only occasionally bound with neighboring particles by small outgrowths. There was no tight interaction of particles inside the implant. In implants formed by pressed particles, cells grow not only on surface, but also in the depth of the implant. Thus, we suppose that a tighter interaction of cells with the titanium implant and, supposedly, tissues with the implant in the organism will take place in the variant when the implant structure is formed by pressed titanium particles, i.e., cellular interaction was observed inside the implant. In implants with irregular pores, cells grew both on the surface and in the depth. Thus, cells exhibited more adequate interactions with irregular pore titanium implants in vitro and hopefully the same interaction will be true in tissues after the implantation of the prosthesis into the organism.

中文翻译：

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各种结构的多孔钛植入物上的细胞培养。


本文介绍了在两种多孔钛植入物上培养人真皮成纤维细胞和兔间充质基质细胞的数据，即由钛颗粒压制形成的不规则孔和由单一尺寸钛颗粒内聚形成的规则孔植入物内部。本研究的目的是确定哪种类型的钛植入物孔隙度可确保其与细胞最强的相互作用。细胞在植入物上培养7天。在此期间，它们在植入物表面形成汇合的单层。通过扫描电子显微镜监测钛植入物上生长的细胞。成纤维细胞与植入物的相互作用取决于植入物的孔隙结构。在具有不规则孔的植入物上，细胞更加分散。在具有规则孔的植入物上，成纤维细胞包裹颗粒，并且仅偶尔通过小生长物与邻近颗粒结合。植入物内部的颗粒没有紧密的相互作用。在由压制颗粒形成的植入物中，细胞不仅在表面生长，而且在植入物的深度生长。因此，我们假设，当植入物结构由压制的钛颗粒形成时，在变体中，细胞与钛植入物以及组织与生物体中的植入物之间将发生更紧密的相互作用，即，在组织内部观察到细胞相互作用。注入。在具有不规则孔的植入物中，细胞在表面和深处都生长。因此，细胞在体外表现出与不规则孔钛植入物更充分的相互作用，并且希望在将假体植入生物体后，在组织中也能出现相同的相互作用。