There are many commercial antibodies with little information provided by their suppliers as to their reliability. Accordingly, commercial antibodies require proper validation before being used in scientific research. In this study, we validated several commercial antibodies, including anti-CSPα, SNAP25, tyrosine hydroxylase, ubiquitin, cleaved caspase 3, and pSer PKC motif. Anti-CSPα, SNAP25, and tyrosine hydroxylase antibodies could detect their endogenous target proteins with some degree of cross-reactivity. Furthermore, clear SNAP25 staining was observed with SNAP25 antibody. Antibodies directed against ubiquitin, cleaved caspase 3, and pSer PKC motif could detect poly-ubiquitination, apoptosis, and phosphorylation, respectively.

中文翻译：

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验证抗CSPα，SNAP25，酪氨酸羟化酶，泛素，切割的半胱天冬酶3和pSer PKC母题抗体是否可在Western印迹中使用。

有许多商业抗体，其供应商很少提供有关其可靠性的信息。因此，商业抗体在用于科学研究之前需要进行适当的验证。在这项研究中，我们验证了几种商业抗体，包括抗CSPα，SNAP25，酪氨酸羟化酶，泛素，裂解的caspase 3和pSer PKC基序。抗CSPα，SNAP25和酪氨酸羟化酶抗体可以检测到它们的内源性靶蛋白，并且具有一定程度的交叉反应性。此外，用SNAP25抗体观察到清晰的SNAP25染色。针对泛素，裂解的半胱天冬酶3和pSer PKC基序的抗体可以分别检测多泛素化，凋亡和磷酸化。