Validation of Anti-CSPα, SNAP25, Tyrosine Hydroxylase, Ubiquitin, Cleaved Caspase 3, and pSer PKC Motif Antibodies for Utilization in Western Blotting

Toshihiko Shirafuji; Takehiko Ueyama; Shigeru Tanaka; Izumi Hide; Naoaki Saito; Norio Sakai

doi:10.1267/ahc.17028

NOTE

Validation of Anti-CSPα, SNAP25, Tyrosine Hydroxylase, Ubiquitin, Cleaved Caspase 3, and pSer PKC Motif Antibodies for Utilization in Western Blotting

Toshihiko Shirafuji, Takehiko Ueyama, Shigeru Tanaka, Izumi Hide, Naoaki Saito, Norio Sakai

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Keywords: validation, antibody, Western blotting

JOURNAL FREE ACCESS FULL-TEXT HTML

2017 Volume 50 Issue 6 Pages 177-180

DOI https://doi.org/10.1267/ahc.17028

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Abstract

There are many commercial antibodies with little information provided by their suppliers as to their reliability. Accordingly, commercial antibodies require proper validation before being used in scientific research. In this study, we validated several commercial antibodies, including anti-CSPα, SNAP25, tyrosine hydroxylase, ubiquitin, cleaved caspase 3, and pSer PKC motif. Anti-CSPα, SNAP25, and tyrosine hydroxylase antibodies could detect their endogenous target proteins with some degree of cross-reactivity. Furthermore, clear SNAP25 staining was observed with SNAP25 antibody. Antibodies directed against ubiquitin, cleaved caspase 3, and pSer PKC motif could detect poly-ubiquitination, apoptosis, and phosphorylation, respectively.

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