The present study investigates the production and partial biochemical characterization of an extracellular thermostable xylanase from the Bacillus oceanisediminis strain SJ3 newly recovered from Algerian soil using three phase partitioning (TPP). The maximum xylanase activity recorded after 2 days of incubation at 37 °C was 20.24 U/ml in the presence of oat spelt xylan. The results indicated that the enzyme recovered in the middle phase of TPP system using the optimum parameters were determined as 50% ammonium sulfate saturation with 1.0:1.5 ratio of crude extract: t-butanol at pH and temperature of 8.0 and 10 °C, respectively. The xylanase was recovered with 3.48 purification fold and 107% activity recovery. The enzyme was optimally active at pH 7.0 and was stable over a broad pH range of 5.0–10. The optimum temperature for xylanase activity was 55 °C and the half-life time at this temperature was of 6 h. At this time point the enzyme retained 50% of its activity after incubation for 2 h at 95 °C. The crude enzyme resist to sodium dodecyl sulfate and β-mercaptoethanol, while all the tested ions do not affect the activity of the enzyme. The recovered enzyme is, at least, stable in tested organic solvents except in propanol where a reduction of 46.5% was observed. Further, the stability of the xylanase was higher in hydrophobic solvents where a maximum stability was observed with cyclohexane. These properties make this enzyme to be highly thermostable and may be suggested as a potential candidate for application in some industrial processes. To the best of our knowledge, this is the first report of xylanase activity and recoverey using three phase partitioning from B. oceanisediminis.

中文翻译：

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利用从海洋芽孢杆菌菌株SJ3提取物中进行的三相分配回收的一种新的热稳定和溶剂稳定的木聚糖酶的生化特性。

本研究调查了使用三相分配（TPP）技术从阿尔及利亚土壤中新分离出的海生芽孢杆菌SJ3菌株产生的细胞外热稳定木聚糖酶的产生及其部分生化特性。在燕麦拼木聚糖的存在下，于37°C孵育2天后记录的最大木聚糖酶活性为20.24 U / ml。结果表明，使用最佳参数在TPP系统中相中回收的酶被确定为50％硫酸铵饱和度，粗提物：t的比例为1.0：1.5。-丁醇的pH值和温度分别为8.0和10°C。回收木聚糖酶，其纯化倍数为3.48，活性回收率为107％。该酶在pH 7.0时具有最佳活性，并且在5.0-10的宽pH范围内都稳定。木聚糖酶活性的最佳温度为55°C，在该温度下的半衰期为6 h。在此时间点，酶在95°C孵育2小时后仍保留其活性的50％。粗酶抵抗十二烷基硫酸钠和β-巯基乙醇，而所有测试离子均不影响酶的活性。回收的酶至少在测试的有机溶剂中稳定，除了在丙醇中观察到的减少量为46.5％。此外，木聚糖酶在疏水性溶剂中的稳定性更高，其中在环己烷中观察到最大的稳定性。这些特性使该酶具有极高的热稳定性，并可能被建议作为在某些工业过程中应用的潜在候选者。据我们所知，这是木聚糖酶活性和回收率的第一个报告，使用了来自B．