Mass spectrometry (MS) is a type of analysis used to determine what molecules make up a sample, based on the mass spectrum that are created by the ions. Mass spectrometers are able to perform traditional target analyte identification and quantitation; however, they may also be used within a clinical setting for the rapid identification of bacteria. The causative agent in sepsis is changed over time, and clinical decisions affecting the management of infections are often based on the outcomes of bacterial identification. Therefore, it is essential that such identifications are performed quickly and interpreted correctly. Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometer is one of the most popular MS instruments used in biology, due to its rapid and precise identification of genus and species of an extensive range of Gram-negative and -positive bacteria. Microorganism identification by Mass spectrometry is based on identifying a characteristic spectrum of each species and then matched with a large database within the instrument. The present review gives a contemporary perspective on the challenges and opportunities for bacterial identification as well as a written report of how technological innovation has advanced MS. Future clinical applications will also be addressed, particularly the use of MALDI-TOF MS in the field of microbiology for the identification and the analysis of antibiotic resistance.

中文翻译：

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MALDI-TOF质谱在临床微生物学中的现状

质谱 (MS) 是一种分析类型，用于根据离子产生的质谱来确定样品中的分子。质谱仪能够执行传统的目标分析物识别和定量；然而，它们也可以在临床环境中用于快速鉴定细菌。脓毒症的病原体会随着时间而改变，影响感染管理的临床决策通常基于细菌鉴定的结果。因此，必须快速执行此类识别并正确解释。基质辅助激光解吸/电离飞行时间 (MALDI-TOF) 质谱仪是生物学中最流行的 MS 仪器之一，由于它可以快速准确地识别广泛的革兰氏阴性和阳性细菌的属和种。质谱鉴定微生物是基于识别每个物种的特征光谱，然后与仪器内的大型数据库匹配。本综述就细菌鉴定的挑战和机遇提供了当代视角，并提供了一份关于技术创新如何推进 MS 的书面报告。未来的临床应用也将得到解决，特别是在微生物学领域使用 MALDI-TOF MS 来识别和分析抗生素耐药性。质谱鉴定微生物是基于识别每个物种的特征光谱，然后与仪器内的大型数据库匹配。本综述就细菌鉴定的挑战和机遇提供了当代视角，并提供了一份关于技术创新如何推进 MS 的书面报告。未来的临床应用也将得到解决，特别是在微生物学领域使用 MALDI-TOF MS 来识别和分析抗生素耐药性。质谱鉴定微生物是基于识别每个物种的特征光谱，然后与仪器内的大型数据库匹配。本综述就细菌鉴定的挑战和机遇提供了当代视角，并提供了一份关于技术创新如何推进 MS 的书面报告。未来的临床应用也将得到解决，特别是在微生物学领域使用 MALDI-TOF MS 来识别和分析抗生素耐药性。