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CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding.
Communications Biology ( IF 5.2 ) Pub Date : 2019-01-31 , DOI: 10.1038/s42003-019-0288-7
Jaindra N Tripathi 1 , Valentine O Ntui 1 , Mily Ron 2 , Samwel K Muiruri 1 , Anne Britt 2 , Leena Tripathi 1
Affiliation  

Presence of the integrated endogenous banana streak virus (eBSV) in the B genome of plantain (AAB) is a major challenge for breeding and dissemination of hybrids. As the eBSV activates into infectious viral particles under stress, the progenitor Musa balbisiana and its derivants, having at least one B genome, cannot be used as parents for crop improvement. Here, we report a strategy to inactivate the eBSV by editing the virus sequences. The regenerated genome-edited events of Gonja Manjaya showed mutations in the targeted sites with the potential to prevent proper transcription or/and translational into functional viral proteins. Seventy-five percent of the edited events remained asymptomatic in comparison to the non-edited control plants under water stress conditions, confirming inactivation of eBSV into infectious viral particles. This study paves the way for the improvement of B genome germplasm and its use in breeding programs to produce hybrids that can be globally disseminated.

中文翻译:


香蕉属 B 基因组中内源香蕉条纹病毒的 CRISPR/Cas9 编辑。克服了香蕉育种的重大挑战。



车前草 B 基因组 (AAB) 中整合内源香蕉条纹病毒 (eBSV) 的存在是杂交育种和传播的主要挑战。由于 eBSV 在胁迫下激活为感染性病毒颗粒,因此具有至少一个 B 基因组的祖先 Musa balbisiana 及其衍生物不能用作作物改良的亲本。在这里,我们报告了一种通过编辑病毒序列来灭活 eBSV 的策略。 Gonja Manjaya 的再生基因组编辑事件显示目标位点发生突变,有可能阻止正确转录或/和翻译成功能性病毒蛋白。在水分胁迫条件下,与未编辑的对照植物相比,75% 的编辑事件保持无症状,这证实了 eBSV 灭活为感染性病毒颗粒。这项研究为 B 基因组种质的改进及其在育种计划中的应用铺平了道路,以生产可在全球传播的杂交种。
更新日期:2019-01-31
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