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Interrelation of Elasticity, Isotherm of Adsorbed Proteins, and its Subsequent Displacement by a Surfactant
Industrial & Engineering Chemistry Research ( IF 3.8 ) Pub Date : 2019-02-14 , DOI: 10.1021/acs.iecr.8b06460 Indu Sharma 1 , Sudip K. Pattanayek 1
Industrial & Engineering Chemistry Research ( IF 3.8 ) Pub Date : 2019-02-14 , DOI: 10.1021/acs.iecr.8b06460 Indu Sharma 1 , Sudip K. Pattanayek 1
Affiliation
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Quartz crystal microbalance with dissipation (QCM-D) is used to study the adsorption characteristics of insulin, myoglobin, bovine serum albumin, and β-galactosidase over amine and methyl surfaces. The water-based and D2O-based phosphate buffers are used for making protein solution. The kinetics of adsorption data, equilibrium adsorption data, and the elasticity of an adsorbed protein is obtained experimentally. A kinetic model of the adsorption of protein with surface phase is developed and fitted to experimental kinetic data. The kinetic parameter involving the first binding step with the surface depends on the surface and physical characteristics of the protein such as charge and component of the secondary structure. The experimental adsorption isotherm data are fitted to a multilayer model with two equilibrium adsorption constants (Ks and KL). The relative strength of adsorption over the bare surface is denoted by Ks, and the relative adsorption strength of protein over the adsorbed protein is denoted by KL. A protein containing a higher amount of β content has a higher Ks value during its adsorption on amine surface. The elastic component G′ of the viscoelastic properties of the adsorbed proteins is found to be high for the protein layer obtained through high Ks and low KL values. The desorption kinetics of the adsorbed proteins using a surfactant solution is also investigated. The elastic properties of the adsorbed proteins and desorption characteristics of the adsorbed proteins by a surfactant (sodium dodecyl sulfate) are strongly related to the adsorption strengths determining factors Ks and KL. An adsorbed protein obtained through a high Ks value is difficult to remove by the surfactant solution.
中文翻译：
弹性，吸附蛋白的等温线及其随后被表面活性剂置换的相互关系
耗散石英晶体微天平（QCM-D）用于研究胰岛素，肌红蛋白，牛血清白蛋白和β-半乳糖苷酶在胺和甲基表面上的吸附特性。水性和D 2O型磷酸盐缓冲液用于制备蛋白质溶液。通过实验获得了吸附数据的动力学，平衡吸附数据和被吸附蛋白质的弹性。建立了表面相吸附蛋白质的动力学模型，并将其拟合到实验动力学数据中。涉及与表面的第一结合步骤的动力学参数取决于蛋白质的表面和物理特性，例如二级结构的电荷和组分。将实验吸附等温线数据拟合到具有两个平衡吸附常数（K s和K L）的多层模型。裸露表面的相对吸附强度用K s表示，蛋白质相对于被吸附蛋白质的相对吸附强度用K L表示。β含量较高的蛋白质在胺表面的吸附过程中具有较高的K s值。对于通过高K s和低K L获得的蛋白质层，发现吸附蛋白质的粘弹性的弹性成分G '较高。价值观。还研究了使用表面活性剂溶液吸附的蛋白质的解吸动力学。表面活性剂（十二烷基硫酸钠）的吸附蛋白的弹性特性和吸附蛋白的解吸特性与决定因子K s和K L的吸附强度密切相关。通过高K s值获得的吸附蛋白难以通过表面活性剂溶液除去。
更新日期：2019-02-14
中文翻译：
弹性，吸附蛋白的等温线及其随后被表面活性剂置换的相互关系
耗散石英晶体微天平（QCM-D）用于研究胰岛素，肌红蛋白，牛血清白蛋白和β-半乳糖苷酶在胺和甲基表面上的吸附特性。水性和D 2O型磷酸盐缓冲液用于制备蛋白质溶液。通过实验获得了吸附数据的动力学，平衡吸附数据和被吸附蛋白质的弹性。建立了表面相吸附蛋白质的动力学模型，并将其拟合到实验动力学数据中。涉及与表面的第一结合步骤的动力学参数取决于蛋白质的表面和物理特性，例如二级结构的电荷和组分。将实验吸附等温线数据拟合到具有两个平衡吸附常数（K s和K L）的多层模型。裸露表面的相对吸附强度用K s表示，蛋白质相对于被吸附蛋白质的相对吸附强度用K L表示。β含量较高的蛋白质在胺表面的吸附过程中具有较高的K s值。对于通过高K s和低K L获得的蛋白质层，发现吸附蛋白质的粘弹性的弹性成分G '较高。价值观。还研究了使用表面活性剂溶液吸附的蛋白质的解吸动力学。表面活性剂（十二烷基硫酸钠）的吸附蛋白的弹性特性和吸附蛋白的解吸特性与决定因子K s和K L的吸附强度密切相关。通过高K s值获得的吸附蛋白难以通过表面活性剂溶液除去。
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