The need for the functionalization of magnetic, water-soluble dyed microspheres with peptides is apparent with the ever-growing biointeraction capabilities and the increased use of dyed microspheres in multiplex, microsphere-based detection assays. This method describes the attachment of any peptide to dyed magnetic microspheres regardless of peptide length, size, or sequence. The method exploits 'click' chemistry with short reaction times in a mixed organic/water system for simultaneous selective surface functionalization and reduction of microsphere dye leaching. All optimization studies were performed using a Luminex 200 assay platform, but the functionalized microspheres are capable of use in any similar multiplex format.

中文翻译：

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一种用肽对染色磁性微球进行功能化的通用方法

随着生物相互作用能力的不断提高和染色微球在基于微球的多重检测分析中使用的增加，显然需要用肽对磁性水溶性染色微球进行功能化。该方法描述了任何肽与染色磁性微球的连接，而不管肽的长度、大小或序列如何。该方法利用在混合有机/水系统中反应时间短的“点击”化学，同时进行选择性表面功能化和减少微球染料浸出。所有优化研究均使用 Luminex 200 分析平台进行，但功能化微球能够以任何类似的多重格式使用。