Lifespan in many organisms, including Drosophila melanogaster, can be increased by reduced insulin-IGF-like signaling (IIS) or by changes in diet. Most studies testing whether IIS is involved in diet-mediated lifespan extension employ only a few diets, but recent data shows that a broad range of nutritional environments is required. Here, we present lifespan data of long-lived Drosophila, lacking three of the eight insulin-like peptides [Drosophila insulin-like peptides 2,3,5 (dilp2-3,5)] on nine different diets that surround the optimum for lifespan. Their nutritional content was varied by manipulating sugar and yeast concentrations independently, and thus incorporated changes in both diet restriction and nutrient balance. The mutants were substantially longer-lived than controls on every diet, but the effects on the lifespan response to sugar and yeast differed. Our data illustrates how a greater coverage of diet balance (DB) and restriction can unify differing interpretations of how IIS might be involved in the response of lifespan to diet.

通过减少胰岛素-IGF样信号传导（IIS）或改变饮食，可以延长许多生物的寿命，包括果蝇（Drosophila melanogaster）。大多数测试IIS是否参与饮食介导的寿命延长的研究仅采用几种饮食，但最新数据表明需要多种营养环境。在这里，我们提供了长寿命果蝇的寿命数据，该果蝇缺乏八种胰岛素样肽中的三种[果蝇胰岛素样肽2,3,5（dilp2-3,5）]九种不同的饮食，这些饮食围绕着寿命的最佳状态。它们的营养含量通过独立地控制糖和酵母的浓度而变化，因此在饮食限制和营养平衡方面都进行了调整。在每种饮食中，突变体的寿命都比对照组长得多，但对糖和酵母的寿命响应的影响却有所不同。我们的数据说明了如何更好地覆盖饮食平衡（DB）和限制饮食可以统一对IIS如何参与寿命对饮食的反应的不同解释。