Periodontitis is an inflammatory autoimmune disease. Treatment should alleviate inflammation, regulate the immune reaction and promote periodontal tissue regeneration. Icariin is the main active ingredient of Epimedii Folium, and it is a promising compound for the enhancement of mesenchymal stem cell function, promotion of bone formation, inhibition of bone resorption, alleviation of inflammation and regulation of immunity. The study investigated the effect of icariin on periodontal tissue regeneration in a minipig model of periodontitis. The minipig model of periodontitis was established. Icariin was injected locally. The periodontal clinical assessment index, a computed tomography (CT) scan, histopathology and enzyme-linked immune sorbent assay (ELISA) were used to evaluate the effects of icariin. Quantitative analysis results 12 weeks post-injection demonstrated that probing depth, gingival recession, attachment loss and alveolar bone regeneration values were (3.72 ± 1.18) mm vs. (6.56 ± 1.47) mm, (1.67 ± 0.59) mm vs. (2.38 ± 0.61) mm, (5.56 ± 1.29) mm vs. (8.61 ± 1.72) mm, and (25.65 ± 5.13) mm³ vs. (9.48 ± 1.78) mm³ in the icariin group and 0.9% NaCl group, respectively. The clinical assessment, CT scan, and histopathology results demonstrated significant enhancement of periodontal tissue regeneration in the icariin group compared to the 0.9% NaCl group. The ELISA results suggested that the concentration of interleukin-1 beta (IL-1β) in the icariin group was downregulated compared to the 0.9% NaCl group, which indicates that local injection of icariin relieved local inflammation in a minipig model of periodontitis. Local injection of icariin promoted periodontal tissue regeneration and exerted anti-inflammatory and immunomodulatory function. These results support the application of icariin for the clinical treatment of periodontitis.

牙周炎是一种炎症性自身免疫性疾病。治疗应减轻炎症，调节免疫反应并促进牙周组织再生。鹰嘴豆素是淫羊Fo的主要活性成分，是增强间充质干细胞功能，促进骨形成，抑制骨吸收，减轻炎症和调节免疫力的有前途的化合物。该研究在小型猪牙周炎模型中研究了大麻素对牙周组织再生的影响。建立了牙周炎的小型猪模型。鹰嘴豆素是局部注射的。使用牙周临床评估指标，计算机断层扫描（CT）扫描，组织病理学和酶联免疫吸附测定（ELISA）来评估icariin的效果。icariin组和0.9％NaCl组分别为³ vs.（9.48±1.78）mm ³。临床评估，CT扫描和组织病理学结果表明，与0.9％NaCl组相比，二十碳黄素组的牙周组织再生显着增强。ELISA结果表明，与0.9％NaCl组相比，icariin组中的IL-1β（IL-1β）浓度被下调，这表明局部注射icariin可以减轻牙周炎小型猪模型中的局部炎症。局部注射icariin可促进牙周组织再生并发挥抗炎和免疫调节功能。这些结果支持了icariin在牙周炎临床治疗中的应用。