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Mathematical modeling of diurnal patterns of carbon allocation to shoot and root in Arabidopsis thaliana.
npj Systems Biology and Applications ( IF 3.5 ) Pub Date : 2019-01-24 , DOI: 10.1038/s41540-018-0080-1 Lisa Küstner 1 , Thomas Nägele 2 , Arnd G Heyer 1
npj Systems Biology and Applications ( IF 3.5 ) Pub Date : 2019-01-24 , DOI: 10.1038/s41540-018-0080-1 Lisa Küstner 1 , Thomas Nägele 2 , Arnd G Heyer 1
Affiliation
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We developed a mathematical model to simulate dynamics of central carbon metabolism over complete diurnal cycles for leaves of Arabidopsis thaliana exposed to either normal (120 µmol m-2 s-1) or high light intensities (1200 µmol m- 2 s-1). The main objective was to obtain a high-resolution time series for metabolite dynamics as well as for shoot structural carbon formation (compounds with long residence time) and assimilate export of aerial organs to the sink tissue. Model development comprised a stepwise increment of complexity to finally approach the in vivo situation. The correct allocation of assimilates to either sink export or shoot structural carbon formation was a central goal of model development. Diurnal gain of structural carbon was calculated based on the daily increment in total photosynthetic carbon fixation, and this was the only parameter for structural carbon formation implemented in the model. Simulations of the dynamics of central metabolite pools revealed that shoot structural carbon formation occurred solely during the light phase but not during the night. The model allowed simulation of shoot structural carbon formation as a function of central leaf carbon metabolism under different environmental conditions without structural modifications. Model simulations were performed for the accession Landsberg erecta (Ler) and its hexokinase null-mutant gin2-1. This mutant displays a slow growth phenotype especially at increasing light intensities. Comparison of simulations revealed that the retarded shoot growth in the mutant resulted from an increased assimilate transport to sink organs. Due to its central function in sucrose cycling and sugar signaling, our findings suggest an important role of hexokinase-1 for carbon allocation to either shoot growth or assimilate export.
中文翻译：
拟南芥芽和根碳分配的昼夜模式的数学模型。
我们开发了一个数学模型来模拟暴露于正常光强度（120 µmol m-2 s-1）或高光强度（1200 µmol m-2 s-1）的拟南芥叶片在完整昼夜周期中的中心碳代谢动态。主要目标是获得代谢物动力学以及芽结构碳形成（具有长停留时间的化合物）的高分辨率时间序列，并将空中器官同化输出到库组织。模型开发包括逐步增加复杂性，以最终接近体内情况。将同化物正确分配到库输出或芽结构碳形成是模型开发的中心目标。结构碳日增益是根据光合固碳总量的日增量计算的，这是模型中实现的结构碳形成的唯一参数。对中心代谢物池动力学的模拟表明，芽结构碳的形成仅发生在光照阶段，而不是在夜间。该模型允许在不同环境条件下模拟芽结构碳形成作为中央叶碳代谢的函数，而无需进行结构修改。对种质 Landsbergecta (Ler) 及其己糖激酶无效突变体 gin2-1 进行了模型模拟。该突变体表现出缓慢的生长表型，尤其是在光强度增加的情况下。模拟比较表明，突变体枝条生长迟缓是由于向库器官的同化物运输增加所致。由于其在蔗糖循环和糖信号传导中的核心功能，我们的研究结果表明己糖激酶-1 在芽生长或同化输出的碳分配中发挥着重要作用。
更新日期：2019-01-24
中文翻译：
拟南芥芽和根碳分配的昼夜模式的数学模型。
我们开发了一个数学模型来模拟暴露于正常光强度（120 µmol m-2 s-1）或高光强度（1200 µmol m-2 s-1）的拟南芥叶片在完整昼夜周期中的中心碳代谢动态。主要目标是获得代谢物动力学以及芽结构碳形成（具有长停留时间的化合物）的高分辨率时间序列，并将空中器官同化输出到库组织。模型开发包括逐步增加复杂性，以最终接近体内情况。将同化物正确分配到库输出或芽结构碳形成是模型开发的中心目标。结构碳日增益是根据光合固碳总量的日增量计算的，这是模型中实现的结构碳形成的唯一参数。对中心代谢物池动力学的模拟表明，芽结构碳的形成仅发生在光照阶段，而不是在夜间。该模型允许在不同环境条件下模拟芽结构碳形成作为中央叶碳代谢的函数，而无需进行结构修改。对种质 Landsbergecta (Ler) 及其己糖激酶无效突变体 gin2-1 进行了模型模拟。该突变体表现出缓慢的生长表型，尤其是在光强度增加的情况下。模拟比较表明，突变体枝条生长迟缓是由于向库器官的同化物运输增加所致。由于其在蔗糖循环和糖信号传导中的核心功能，我们的研究结果表明己糖激酶-1 在芽生长或同化输出的碳分配中发挥着重要作用。
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