Pseudomonas bacteriocin syringacin M released upon desiccation suppresses the growth of sensitive bacteria in plant necrotic lesions.,Microbial Biotechnology

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Pseudomonas bacteriocin syringacin M released upon desiccation suppresses the growth of sensitive bacteria in plant necrotic lesions.
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2019-01-22 , DOI: 10.1111/1751-7915.13367
Jun-Zhou Li ₁ , Li-Ying Zhou ₁ , You-Liang Peng _{1,

2} , Jun Fan _{1,

3}

Affiliation

Bacteriocins are regarded as important factors mediating microbial interactions, but their exact role in community ecology largely remains to be elucidated. Here, we report the characterization of a mutant strain, derived from Pseudomonas syringae pv. tomato DC3000 (Pst), that was incapable of growing in plant extracts and causing disease. Results showed that deficiency in a previously unannotated gene saxE led to the sensitivity of the mutant to Ca²⁺ in leaf extracts. Transposon insertions in the bacteriocin gene syrM, adjacent to saxE, fully rescued the bacterial virulence and growth of the ΔsaxE mutant in plant extracts, indicating that syrM‐saxE encode a pair of bacteriocin immunity proteins in Pst. To investigate whether the syrM‐saxE system conferred any advantage to Pst in competition with other SyrM‐sensitive pathovars, we compared the growth of a SyrM‐sensitive strain co‐inoculated with Pst strains with or without the syrM gene and observed a significant syrM‐dependent growth reduction of the sensitive bacteria on plate and in lesion tissues upon desiccation–rehydration treatment. These findings reveal an important biological role of SyrM‐like bacteriocins and help to understand the complex strategies used by P. syringae in adaptation to the phyllosphere niche in the context of plant disease.

中文翻译：

干燥后释放的 Pseudomonas bacteriocin syringacin M 可抑制植物坏死病变中敏感细菌的生长。

细菌素被认为是介导微生物相互作用的重要因素，但它们在群落生态学中的确切作用在很大程度上仍有待阐明。在这里，我们报告了来自丁香假单胞菌pv 的突变菌株的特征。番茄DC3000 ( Pst )，不能在植物提取物中生长并引起疾病。结果表明，先前未注释的基因saxE的缺陷导致突变体对叶提取物中的 Ca ^2+敏感。与saxE相邻的细菌素基因syrM中的转座子插入，完全挽救了ΔsaxE的细菌毒力和生长。植物提取物中的突变体，表明syrM-saxE在Pst中编码一对细菌素免疫蛋白。为了研究syrM-saxE系统是否赋予Pst与其他 SyrM 敏感病理变种竞争的任何优势，我们比较了与具有或不具有syrM基因的Pst菌株共同接种的 SyrM 敏感菌株的生长，并观察到显着的syrM-干燥-补液处理后平板和病变组织中敏感细菌的依赖性生长减少。这些发现揭示了 SyrM 样细菌素的重要生物学作用，并有助于理解丁香假单胞菌使用的复杂策略在植物病害背景下适应叶际生态位。

更新日期：2019-01-22

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