The main objective of the study is to investigate the effect of hydraulic retention times on continuous dark fermentative biohydrogen production in an up-flow packed bed reactor (UPBR) containing a novel microorganism immobilization material namely polyester fiber beads. The hydrogen producing dark fermentative microorganisms were obtained by heat-pretreatment of anaerobic sludge from the acidogenic phase of an anaerobic wastewater treatment plant. Glucose was the sole carbon source and the initial concentration was 15 ± 1 g/L throughout the continuous feeding. UPBR was operated under the thermophilic condition at T = 48 ± 2 °C and at varying HRTs between 2 h and 6 h. The hydrogen productivity of continuously operated UPBR increased with increasing HRT. Hydrogen production volume varied between 4331 and 6624 ml/d, volumetric hydrogen production rates (VHPR) were obtained as 3.09–4.73 L H₂/L day, and hydrogen production yields (HY) were 0.49 mol/mol glucose-0.89 mol/mol glucose depending on HRT. Maximum daily hydrogen volume (6624 ml/d), the yield (0.89 mol/mol glucose) and VHPR (4.73 L H₂/L day) were obtained at HRT = 6 h. The production rate and the yield decreased with increasing organic loading rate due to substrate inhibition.

该研究的主要目的是研究水力停留时间对含有新型微生物固定材料即聚酯纤维珠的上流式填充床反应器（UPBR）中连续黑暗发酵生物氢生产的影响。通过从厌氧废水处理厂的产酸阶段对厌氧污泥进行热处理，获得了产氢的深色发酵微生物。葡萄糖是唯一的碳源，在整个连续进料过程中初始浓度为15±1 g / L。UPBR在T = 48±2°C的高温条件下，在2 h和6 h之间变化的HRT下运行。连续运行的UPBR的氢气生产率随着HRT的增加而增加。制氢量在4331和6624 ml / d之间变化，₂ / L天，取决于HRT，氢气产生产率（HY）为0.49 mol / mol葡萄糖-0.89 mol / mol葡萄糖。在HRT = 6 h时获得最大每日氢气量（6624 ml / d），产率（0.89 mol / mol葡萄糖）和VHPR（4.73 LH ₂ / L天）。由于底物抑制，产率和产率随着有机负载率的增加而降低。