The culturable bacteria from root nodules of Sulla coronaria growing in spontaneous conditions in Sardinia were characterized. This plant’s peculiarity is to represent a legume still found in both wild and cropped statuses. We tested whether culturable bacteria would differ from those commonly isolated from its field-cropped varieties, to date exclusively represented by Rhizobium sullae. 63 isolates from 60 surface-sterilized nodules were analyzed by ARDRA and 16S rDNA sequencing. The official nitrogen-fixing symbiont Rhizobium sullae was found only in 25 nodules out of 60. The remaining nodules did not yield culturable rhizobia but a number of different endophytic genera including Pseudomonas sp. (17 nodules), Microbacterium sp. (15 nodules), Pantoea agglomerans (5 nodules). The situation appears therefore a hybrid between what is commonly observed in other Mediterranean legumes occurring only in wild status (featuring non-culturable rhizobia and arrays of culturable endophytes within nodules), as opposed to cropped legumes (endowed with fully culturable rhizobia and minimal endophytic occurrence). These findings, within a species bridging the ecology between native and cropped conditions, suggest insights on the relative importance of endophytic co-occupancy vs. true N-fixing symbiont culturability within nodules. The latter trait thus appears to accompany the domestication path of plants with a main trade-off of renouncing to interactions with a diversity of endophytic co-invaders; the relationships with those being critical in the non-cropped status. In fact, endophytes are known to promote plant growth in harsh conditions, which can be particularly stressful in the Mediterranean due to drought, highly calcareous soils, and pathogens outbreaks.

从撒丁岛自发条件下生长的冠状苏拉根瘤的可培养细菌进行了表征。这种植物的独特之处在于代表了一种在野生和种植状态下都仍然可以发现的豆科植物。我们测试了可培养细菌是否与通常从田间种植的品种中分离出来的细菌有所不同，迄今为止，这些细菌仅由沙哑根瘤菌代表。通过ARDRA和16S rDNA测序分析了来自60个表面灭菌结节的63个分离株。官方固氮共生根瘤菌sullae仅见于25个结节出来的60.剩下的结节没有屈服培养的根瘤菌，但许多不同的内生属，包括假单胞菌属。（17个结节），微杆菌属。（15个结节），聚结泛菌（5个结节）。因此，这种情况似乎是在其他地中海豆科植物中常见的现象之间的混合，仅在野生状态下出现（具有不可培养的根瘤菌和根瘤内可培养的内生菌的特征），与农作物豆类（具有完全可培养的根瘤菌和最小的内生菌发生）相反）。这些发现在一个在自然条件和耕作条件之间架起桥梁的物种中，提出了关于内生共生与结核内真正的固氮共生体可培养性的相对重要性的见解。因此，后一种性状似乎伴随着植物的驯化路径，主要的折衷是放弃与多种内生共入侵物的相互作用。与那些在非裁剪状态下至关重要的关系。实际上，